| 白小军，王宪国，陈东升.宁夏小麦品种慢锈基因 Lr34/Yr18的分子检测[J].麦类作物学报,2014,34(11):1480 |
| 宁夏小麦品种慢锈基因 Lr34/Yr18的分子检测 |
| Molecular Detection of the Slow-Rusting Resistance Gene Lr34/Yr18 in Ningxia Wheat Cultivars |
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| DOI：10.7606/j.issn.1009-1041.2014.11.05 |
| 中文关键词: 普通小麦 Lr34/Yr18基因 STS 标记 csLV34 |
| 英文关键词:Common wheat(Triticum aestivum L.) Lr34/Yr18 STS marker csLV34 |
| 基金项目:宁夏农业育种专项“小麦新品种选育”；宁夏科技支撑计划（国际科技合作）项目（2013ZYH196）；宁夏农林科学院自主研发项目（NKYG-13-02）；宁夏自然科学基金项目（NZ13108）；西北农林科技大学唐仲英育种基金项目 |
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| 摘要点击次数: 2008 |
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| 中文摘要: |
| Lr34/Yr18是重要的小麦慢叶锈/慢条锈基因，可用于小麦锈病抗性改良。为了明确 Lr34/Yr18基因在宁夏小麦中的分布特点，利用STS标记 csLV34 对111份宁夏小麦品种中慢锈基因 Lr34/Yr18的等位变异进行了分子检测，并且进行了成株期条锈病抗性鉴定。结果表明，20份材料携带 Lr34/Yr18基因，占18.0%。不同来源的小麦品种中 Lr34/Yr18基因分布频率不同，农家品种中分布频率最高，占90.9%；引进品种中所占比例为14.3%；育成品种中所占比例最低，仅占7.7%。含有 Lr34/Yr18基因的品种对条锈病具有较好的抗性，可作为今后宁夏小麦抗锈病育种的重要抗源。 |
| 英文摘要: |
| Lr34 and Yr18 are important slow-rusting resistance genes. Characterizing the distribution of Lr34/Yr18 will benefit the improvement of rust resistance in Ningxia wheat breeding program. The presence of Lr34/Yr18 gene was detected in 111 Ningxia wheat cultivars and landraces using an STS marker csLV34 , which could amplify a 150 bp and a 229 bp fragment in the genotypes with and without Lr34/Yr18, respectively. The results indicated that only 20 genotypes carried the resistant gene Lr34/Yr18 among the Ningxia wheat materials, accounting for 18.0%. Different frequencies were found in landraces, introduced cultivars, and improved cultivars, which were 90.9%, 14.3% and 7.7%, respectively. These varieties with Lr34/Yr18 genes showed good resistance to stripe rust and could be used in wheat breeding targeting for stripe rust and leaf rust in Ningxia. |
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