| 原红军，曾兴权，王玉林，徐齐君，韦泽秀，尼玛扎西.青稞法尼基转移酶β亚基编码基因 HbERA1 的克隆及表达分析[J].麦类作物学报,2014,34(11):1465 |
| 青稞法尼基转移酶β亚基编码基因 HbERA1 的克隆及表达分析 |
| Cloning and Characterization of Beta Subunit of Protein Farnesyl Transferase ERA1 in Tibetan Hulless Barley (Hordeum vulgare subsp. vulgare) |
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| DOI：10.7606/j.issn.1009-1041.2014.11.03 |
| 中文关键词: 青稞 HbERA1 基因克隆 干旱胁迫 表达模式 |
| 英文关键词:Hordeum vulgare subsp. vulgare HbERA1 Gene cloning Drought Stress Expression patterns |
| 基金项目:国家重点基础研究计划（973计划）前期研究专项（2012CB723006）；国家科技支撑计划项目（2012BAD03B01;2013BAD30B01）；西藏财政专项（2014CZZ001） |
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| 摘要点击次数: 1993 |
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| 中文摘要: |
| ERA1 （Enhanced response to ABA）基因编码法尼基转移酶（Farnesyl transferase）β亚基，该酶在干旱胁迫下对ABA信号负向调控因子的修饰起着关键作用。本研究以青稞（Hordeum vulgare subsp. vulgare）抗旱品种喜马拉雅10号为材料，利用RT-PCR技术克隆获得了 ERA1 基因全长cDNA序列，命名为 HbERA1 （登录号：KJ699392）。生物信息学分析表明，该基因全长1 401 bp，可编码466个氨基酸序列，蛋白分子量为51.14 kD，等电点为5.00。Prosite Scan分析结果表明， HbERA1 含有多个干旱胁迫响应蛋白的作用位点，如酪蛋白激酶Ⅱ磷酸化位点、N-糖基化位点、蛋白激酶C磷酸化位点及N-豆蔻酰化位点。利用实时定量PCR方法研究了 HbERA1 在干旱胁迫条件下及复水后不同时间点的表达情况，发现在水分过剩处理下（土壤绝对含水量>15.5%）， HbERA1 在土壤绝对含水量为33.4%时表达量最高，并随着土壤绝对含水量的下降而下调表达；进行干旱胁迫后（<15.5%）基因表达量也明显下调表达；复水后表达逐渐恢复，复水8 h时超过正常表达水平，表明 HbERA1 基因可能参与调控水涝和干旱胁迫双重信号传导。 |
| 英文摘要: |
| Drought stress has become one of the factors that hamper the development of modern agriculture. Varieties with enhanced drought resistance can be an effective way to solve this problem. Enhanced response to abscisic acid 1 ( ERA1 ) gene encoded the beta subunit of farnesyl transferase, which had been thought to be a negative regulator for ABA signal transduction. However, the exact mechanism underlining is still to be elusive. In this manuscript, we presented the isolation of a new ERA1 gene from Hordeum vulgare subsp. vulgare, nominated as HbERA1 (Accession No. Kj699392) by RT-PCR, which were proved to be highly drought tolerance. The gene was 1401 bp in length, encoding a peptide of 466 amino acids, molecular weight about 51.14 kD and with pI of 5.00.Results from Prosite Scan indicated that HbERA1 contains multiple domains of drought stress response cis-element such as casein Kinase II phosphorylation sites, n-glycosylation sites, protein kinase c-phosphorylation and n-cardamom acylation sites. Expression patterns of HbERA1 were investigated by RT-qPCR at serious waterlogging or drought stress and at various time points after recovery:At the water logging level (>15.5% of absolute moisture),the highest expression level was observed in plants growing in soil absolute moisture 33.4%, which may brought waterlogging to tibet barley. As soil absolute moisture declined, its transcripts decreased sharply. When plants were drought stressed (<15.5% of absolute moisture), the expression of this gene was repressed again. After recovery from drought stress, the expression increased and the plants possessed a relative higher expression level of HbERA1 compared to normal plants,after an 8 h recovery from drought stress. It indicated that HbERA1 gene played a role in regulating for both waterlogging and drought stress. |
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