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周渭皓,孙建喜,陈 杰,陈 锋,崔党群.甘肃春小麦八氢番茄红素基因的等位变异[J].麦类作物学报,2014,34(8):1036
甘肃春小麦八氢番茄红素基因的等位变异
Allelic Variants of Phytoene Synthase 1 ( Psy 1) Genes in Spring Wheat Cultivars from Gansu
  
DOI:10.7606/j.issn.1009-1041.2014.08.03
中文关键词:  春小麦  黄色素含量  八氢番茄红素合成酶基因  等位基因变异
英文关键词:Spring wheat  Yellow pigment content  Phytoene synthase gene  Allelic variation
基金项目:教育部新世纪优秀人才项目(NCET 13 0776);河南省高校科技创新团队和河南省高校科技创新人才项目(2012HASTIT007)
作者单位
周渭皓,孙建喜,陈 杰,陈 锋,崔党群 (1.河南农业大学农学院/河南省粮食作物协同创新中心/小麦玉米作物学国家重点实验室河南郑州 4500022.驻马店市农科院河南驻马店 463000) 
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中文摘要:
      为研究我国小麦面粉色泽形成的分子机理,利用功能标记 YP7A、YP7A 2、YP7B 1、YP7B 2、YP7B 3、YP7B 4、YP7D 1 YP7D 2对来自我国甘肃省的62份春小麦主栽品种 Psy1位点的等位变异类型进行了检测,并对其黄色素含量进行了测定。结果表明,在Psy A1位点共发现有 Psy A1aPsy A1b两种基因型,其频率分别为64.52%和35.48%;在Psy B1位点共发现有 Psy B1aPsy B1bPsy B1cPsy B1d 四种等位变异类型,其分布频率分别为54.84%、38.71%、4.84%和1.61%;在Psy D1位点除墨引1号没有扩增条带之外,其余材料基因型均为Psy D1a。进一步分析表明,参试材料中共有7种基因型组合,分别为 Psy A1a/ Psy B1a/Psy D1aPsy A1a/Psy B1b/Psy D1aPsy A1b/Psy B1a/Psy D1aPsy A1a/Psy B1c/Psy D1aPsy A1b/Psy B1b/Psy D1aPsy A1a/Psy B1d/Psy D1aPsy A1b/Psy B1c/Psy D1a,其分布频率分别为33.87%、25.81% 20.97%、12.9%、3.23%、1.61%和1.61%。其中,拥有 Psy A1a/Psy B1c/Psy D1a基因型组合的小麦品种黄色素含量显著高于其他基因型组合,而拥有 Psy A1b/Psy B1b/Psy D1a基因型组合的小麦品种黄色素含量显著低于其他基因型组合。本研究能够为我国春小麦面粉色泽的遗传改良提供重要信息。
英文摘要:
      In order to probe into the molecular mechanisms for flour color of China wheat,functional markers of YP7A,YP7A 2,YP7B 1,YP7B 2,YP7B 3,YP7B 4,YP7D 1 and YP7D 2 were used to identify allelic variations of Psy 1 genes,and their associations with yellow pigment content were analyzed in 62 spring wheat cultivars from Gansu.Two alleles Psy A1a and Psy A1b were observed at Psy A1 locus and their percentages were 64.52% and 35.48%,respectively.Four alleles Psy B1a,Psy B1b,Psy B1c and Psy B1d were found at Psy B1 locus and their percentages were 54.84%,38.71%,4.84% and 1.61%,respectively.All cultivars possessed Psy D1a allele at Psy D1 locus except for Moyin 1.Furthermore,seven allelic combinations of Psy 1 genes were identified in wheat cultivars surveyed,i.e., Psy A1a/Psy B1a/Psy D1a, Psy A1a/Psy B1b/Psy D1a,Psy A1b/ Psy B1a/Psy D1a,Psy A1b/Psy B1b/Psy D1a, Psy A1a/Psy B1c/Psy D1a, Psy A1a/Psy B1d/Psy D1a and Psy A1b/Psy B1c/Psy D1a, and their percentages were 33.87%,25.81%,20.97%,12.90%,3.23%,1.61% and 1.61%,respectively.Analysis of association of Psy 1 allelic variations with yellow pigment content indicated that wheat cultivars with Psy A1a/Psy B1c/Psy D1a possessed significantly higher yellow pigment content,whereas wheat cultivars with Psy A1b/Psy B1b/Psy D1a possessed significantly lower yellow pigment content among the seven Psy 1 allelic combinations.These results could provide useful information for the improvement of flour color in Chinese spring wheat.
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