| NRT1 and NRT2 families play important roles in the response to nitrogen starvation in higher plants. In order to explore the roles of wheat NRT1 and NRT2 genes in response to N starvation, the contents of chlorophyll, nitrates, protein and malondialdehyde (MDA) were measured in the leaves of N starved wheat seedlings. The NRT1 (7) and NRT2 (5) gene families from common wheat were identified and their transcription levels were further measured using quantitative real time PCR (qPCR) method in leaves of N starved wheat seedlings. The results indicated that nitrogen starvation significantly inhibited the growth of wheat seedlings, manifested by decreased plant heights, total fresh weights, and total dry weights. During N starvation period, contents of chlorophyll, nitrates and protein were also remarkably reduced, whereas the contents of malondialdehyde (MDA) were markedly increased in leaves of wheat seedlings. N starvation greatly inhibited the transcription levels of TaNRT1.1, TaNRT1.2, TaNRT1.7, TaNRT2.3 and TaNRT2.4 genes during all sampling time points (2 d, 4 d, 6 d, and 8 d after N starvation). However, N starvation significantly enhanced the transcript levels of TaNRT1.4, TaNRT1.8 and TaNRT2.1 genes at 2 d after N starvation, and induced the transcript levels of TaNRT1.3, TaNRT1.5 and TaNRT2.5 at 4 d after N starvation, implying that TaNRT1.3, TaNRT1.4, TaNRT1.5, TaNRT1.8, TaNRT2.1, and TaNRT2.5 might play important roles in responses of wheat seedlings to nitrogen starvation. |
基本信息
