| 马建华，赵纯钦，徐登安，余懋群，陈 静.两个小麦品种开花相关基因表达的温度效应分析[J].麦类作物学报,2014,34(7):899 |
| 两个小麦品种开花相关基因表达的温度效应分析 |
| Effect of Temperature on Expression of Flowering Time Related Genes in Two Wheat Cultivars |
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| DOI：10.7606/j.issn.1009-1041.2014.07.04 |
| 中文关键词: 小麦 开花时间 基因表达 定量PCR |
| 英文关键词:Triticum aestivum Flowering time Gene expression Real time quantitative PCR |
| 基金项目:中国科学院院地合作项目(XBCD 2011 019）；中国科学院知识创新工程重要方向项目( KSCX2 EW J 22) ；四川省科技厅国际合作项目(2012HH0008)；四川省育种攻关项目 |
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| 中文摘要: |
| 小麦开花受多种遗传途径和环境因素的共同调控。VRN1、FT（开花正向调节因子）和VRN2（开花抑制因子）是主要的开花调控基因。为了解环境温度变化影响小麦开花的分子机制，采用荧光定量PCR方法，分析两个小麦品种川育12和新春9号的开花基因表达量变化与开花时间的关系。结果表明，长日照条件下，小麦品种间开花的温度敏感性不同，川育12的开花时间随环境温度升高提前天数更多。受试品种VRN1与FT表达量呈协同增高的变化趋势，而与整体水平较低的VRN2呈相互消长的变化关系。总体上，川育12的VRN1、FT表达量高于新春9号，VRN2的表达量降幅则低于新春9号。环境温度升高导致品种VRN1、 FT表达进一步增强，且在川育12中的增幅更大。在15 ℃环境温度下，两个品种VRN1和FT表达水平的最大差异倍数分别为7.36和1.95， 24 ℃环境温度下分别为5.51和5.1，表明品种间VRN1、 FT表达的温度敏感性不同。川育12早开花特性与其VRN1、 FT基础表达水平较高以及更强的温度敏感性有关。 |
| 英文摘要: |
| Various genetic pathways and environment factors are integrated to regulate plant seasonal flowering responses. the genes VRN1, FT (positive regulator of flowering) & VRN2 (negative regulator of flowering) are major components of the molecular pathways controlling flowering time in wheat. To investigate the molecular mechanism underlying environment temperature regulation on wheat flowering time, A real time quantitative PCR was used to discover changes in the expression of the key flowering related genes in two wheat varieties (Chuanyu 12 & Xinchun 9), and their connection with flowering time. The results showed, different thermo sensitive flowering responses existed between cultivars under long day conditions, with much earlier flowering in Chuanyu 12 than in Chuanyu 9 after environment temperature rising. Under either relatively low or high temperature (15 ℃/24 ℃), the expressions of VRN1 and FT in both cultivars increased synchronously, whereas VRN2 kept low expression level and decreased gradually towards flowering. Roughly, the expression levels of VRN1 and FT in Chuanyu 12 were much higher than that in Xinchun 9, while the expression levels of VRN2 reducing more quickly in Xinchun 9. The expressions of VRN1 & FT in two cultivars enhanced further when the environment temperature rose, with much more increase in Chuanyu 12. Under 15 ℃ temperature, the maximum expression changes of VRN1 and FT were 7.36 and 1.95 times between two cultivars, respectively, which was 5.51 and 5.1 times under 24 ℃ temperature. It was concluded that high temperature caused the simultaneous increase in expression of VRN1 and FT, and Chuanyu 12 displayed an early flowering trait probably due to the higher basic level and more intense thermo sensitive response of the two genes expression in it. |
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