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简俊涛,田芳慧,张兆萍,朱传杰,刘路平,孙道杰.366份小麦品种春化光周期基因型组成及其与生殖物候稳定性的关系[J].麦类作物学报,2014,34(7):892
366份小麦品种春化光周期基因型组成及其与生殖物候稳定性的关系
Relationship between Combination of Vernalization and Photoperiod Genotypes and the Stability of Reproductive Phenology Based on Study of 366 Wheat Varieties
  
DOI:10.7606/j.issn.1009-1041.2014.07.03
中文关键词:  小麦  春化基因  光周期基因  生殖物候稳定性
英文关键词:Triticum aestivum  Vernalization gene  Photoperiod gene  Reproductive phenology stability
基金项目:国家重点基础研究发展计划(973计划)项目(2014CB138100);国家高技术研究发展计划(863计划)项目(2012AA101105);国际科技合作项目(2012DFA32290);国家自然科学基金项目(31071408)
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简俊涛,田芳慧,张兆萍,朱传杰,刘路平,孙道杰 (1. 西北农林科技大学农学院陕西杨凌 712100 2.濮阳市台前县农业局河南台前 457600) 
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中文摘要:
      为探究国内小麦品种春化光周期基因的显隐性组成及其与生殖物候稳定性的关系,以国内各大麦区主栽小麦品种及近几年的区试品系共366份为材料,采用STS标记对春化基因 Vrn A1、Vrn B1、Vrn D1、Vrn B3和光周期基因 Ppd D1进行检测;同时采用分期播种法,以不同播期之间的抽穗期相差天数为生殖物候稳定性的评价指标,探索春化、光周期基因型对生殖物候稳定性的影响。结果表明,366份材料中包含15种春化光周期基因型组合,其中组合 vrn A1+vrn B1+vrn D1+ Ppd D1a最多,达48.91%,其次为 vrn A1+vrn B1+Vrn D1+ Ppd D1a,占16.39%。 vrn A1+vrn B1+vrn D1+ Ppd D1a能最大程度地维持小麦生殖物候稳定性,使抽穗期差异维持在5.43 d,而 vrn A1+vrn B1+Vrn D1+ Ppd D1b、vrn A1+Vrn B1+Vrn D1+ Ppd D1a、 Vrn A1+vrn B1+Vrn D1+ Ppd D1b则赋予小麦极不稳定的生殖物候特性,使抽穗期差异达10 d以上。此外,显性春化基因能降低小麦生殖物候稳定性,其效应表现为 Vrn A1>Vrn B1>Vrn D1;隐性春化基因能够增强小麦生殖物候稳定性,其效应为vrn B1>vrn D1>vrn A1;光周期基因对生殖物候稳定性影响不显著。
英文摘要:
      In order to make a thorough investigation on the relationship between the combination of dominant or recessive vernalization and photoperiod genes of wheat varieties and the stability of reproductive phenology, a total of 366 wheat cultivars were used as materials in this study, including the major wheat cultivars from the major wheat growing areas in China as well as strains in the regional trial in recent years. STS markers were utilized to detect vernalization genes of Vrn A1, Vrn B1, Vrn D1 and Vrn B3 and photoperiod gene Ppd D1. With method of sowing at two different dates, the number of difference of heading date, based on different sowing dates, were used as evaluation criteria for the stability of reproductive phenology. The results showed that, among the 366 wheat materials, there existed 15 kinds of genotypic combination of vernalization and photoperiod genes, among which vrn A1+vrn B1+vrn D1+ Ppd D1a was the largest group, as 48.91% of the total; and vrn A1+vrn B1+Vrn D1+ Ppd D1a was the second, with 16.39% of total. While the combination of vrn A1+vrn B1+vrn D1+ Ppd D1a could maintain the stability of reproductive phenology of wheat to the greatest extent, with the difference of heading date at about 5.43 days; however, the combinations of vrn A1+vrn B1+Vrn D1+ Ppd D1b, vrn A1+Vrn B1+Vrn D1+ Ppd D1a and Vrn A1+vrn B1+Vrn D1+ Ppd D1b made extremely unstable reproductive phenology characteristics of wheat, with the difference of heading stage discrepancy more than 10 days. Furthermore, dominant vernalization genes could weaken the stability of wheat reproductive phenology, the effects of which demonstrated as that Vrn A1>Vrn B1>Vrn D1;while on the contrary, recessive vernalization genes could intensify the stability of wheat reproductive phenology, which was reflected as that vrn B1>vrn D1>vrn A1. Photoperiod genes did't have obvious impact on reproductive phenology stability.
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