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李天亚,吴限鑫,王 浩,陈 思,曹远银.我国燕麦秆锈菌生理小种与毒力分析[J].麦类作物学报,2014,34(4):552
我国燕麦秆锈菌生理小种与毒力分析
The Race and Virulence Patterns of Puccinia graminis f. sp. avenae in China
  
DOI:10.7606/j.issn.1009-1041.2014.04.19
中文关键词:  燕麦秆锈菌  生理小种  毒力  抗病基因
英文关键词:Puccinia graminis f.sp. avenae  Physiological race  Virulence patterns  Resistance gene
基金项目:国家重点基础研究计划(973计划)项目(2013CB127700);国家公益性行业(农业)科研专项(200903035);国家自然科学基金项目(31171829)。
作者单位
李天亚,吴限鑫,王 浩,陈 思,曹远银 (沈阳农业大学植物保护学院辽宁沈阳 110866) 
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中文摘要:
      为了解当前我国燕麦秆锈菌生理小种及其毒力现状,利用12个北美燕麦秆锈菌单基因鉴别寄主,对2013年采集的7份和2012保存的4份燕麦秆锈病标样进行小种与毒力鉴定,并用Pg a code字母命名系统命名小种,以16个单基因系测定毒力频率。结果表明,从11份燕麦秆锈菌标样中分离成活了26个单孢子堆菌株,鉴定出TKR、TJM和TKM三个生理小种,出现频率分别为61.6%、30.8%和7.6%。26个菌株对 Pg 6 Pg 15无毒力,对 Pg 10 Pg 13Pg a和Rodney 0的毒力频率分别为69.2%、61.6%、61.6%和84.6%,对 Pg 1 Pg 2 Pg 3 Pg 4 Pg 8 Pg 9 Pg 12 Pg 16和Marvellous毒力频率高达100%。说明当前我国燕麦秆锈菌优势小种为TKR,次优势小种为TKM; Pg 6 Pg 15为有效抗病基因, Pg 1 Pg 2 Pg 3 Pg 4 Pg 8 Pg 9 Pg 12 Pg 16和Marvellous则为无效抗病基因, Pg 10 Pg 13Pg a和Rodney 0对我国燕麦秆锈菌具有一定的区分作用。
英文摘要:
      To unveil the current physiological races and virulence patterns of Puccinia graminis f.sp.avenae and to provide guidance for screening of germplasm for stem rust resistance and setting goals of resistance breeding, races of P.graminis f.sp.avenae in China were characterized. 12 single Pg gene differentials of oat used in North America were applied for race identification at seedling stage using Pg a samples collected in oat growing areas in China in 2013. The three letter Pg a code nomenclature system was followed to name the races. The results showed that 26 isolates isolated from 11 accessions were identified as 3 races, namely TKR, TJM and TKM. Their occurrence frequencies were 61.6%, 30.8% and 7.6 %, respectively. It was also indicated that virulence frequency on both Pg 6 and Pg 15was zero, but on Pg 1, Pg 2, Pg 3, Pg 4, Pg 8, Pg 9, Pg 12, Pg 16 and Marvellous, were up to 100% and on Pg 10, Pg 13, Pg a and Rodney 0, was up to 69.2%, 61.6%, 61.6% and 84.6% ,respectively. Three races were found out in the P.graminis f.sp.avenae population from China. Race TKR was the most predominant and the resistance of Pg 6 and Pg 15were highly effective to all of the above races of P.graminis f.sp.avenae.
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