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作者	单位
马丽，李治，任天恒，唐宗祥，晏本菊，任正隆	（1.四川农业大学生命科学与理学院成都教学部,四川成都 611130；2.四川农业大学植物遗传育种省级重点实验室，四川成都 611130)

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中文摘要:

为了鉴定我国西南冬麦区普通小麦品种的穗发芽抗性，筛选能鉴定穗发芽抗性的相关分子标记，利用小麦品种川农17和新品系R146构建的重组自交系（F_7：8）共135个家系作为研究材料，通过测定种子的发芽指数和降落值来共同鉴定小麦的穗发芽抗性。选择7个已发表的与穗发芽抗性相关的分子标记（ Xgwm46、 Xgwm269、 Xgwm282、 Xgwm328、 Xgwm397、 Xwmc468和 Xgwm518）对这些材料进行PCR扩增，分析扩增片段与发芽指数和降落值的相关性。结果表明，在135份重组自交系材料中，发芽指数小于0.4的材料有21份，介于0.4～0.8的材料有86份，高于0.8的材料有28份；降落值小于250的材料有20份，大于400的有16份。标记扩增片段与发芽指数和降落值相关分析表明，7个标记中有3个标记（ Xgwm397、 Xgwm282和 Xwmc468）与穗发芽抗性相关，标记 Xgwm397 和 Xwmc468可作为穗发芽抗性选择的分子标记在育种中加以利用；另外三个标记 Xgwm269、 Xgwm328、 Xgwm518与穗发芽抗性不相关；标记 Xgwm46只与发芽指数相关，与降落值相关不显著，能否用作穗发芽筛选标记需进一步验证。

英文摘要:

To identify the breeding materials with pre harvest sprouting (PHS) tolerance and to screen the efficient markers associated with PHS tolerance, in the study, the germination index and falling number were measured in a set of 135 recombinant inbred lines (F_7:8) which derived from a cross between Chuannong 17 (CN17) and R146, and were used to analyze their correlations with the PCR amplification patterns of seven makers ( Xgwm46， Xgwm269， Xgwm282， Xgwm328， Xgwm397， Xwmc468 and Xgwm518) associated with PHS tolerance. The results showed that among the 135 recombinant inbred lines tested, the germination index of 21 lines were lower than 0.4, that of 86 lines were between 0.4 and 0.8, and 28 lines were over 0.8; the falling number of 20 lines were lower than 250, that of 16 lines were over 400. The relationship between the variations of PCR products of the seven makers with the germination index and falling number of the tested lines confirmed that three markers of Xgwm397, Xgwm282, Xwm468 were associated with PHS response, but that of Xgwm269, Xgwm328, Xgwm518 were not, and whether the maker Xgwm46 was associated with PHS response needed further study.

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