| 李孟军，杨 煜，高欣娜，郭 晓，何明琦，史占良，郭进考.石麦15细菌人工染色体文库构建与鉴定[J].麦类作物学报,2014,34(2):164 |
| 石麦15细菌人工染色体文库构建与鉴定 |
| Construction and Characterization of BAC Library from Common Wheat Shimai 15 |
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| DOI：10.7606/j.issn.1009-1041.2014.02.04 |
| 中文关键词: 小麦 细菌人工染色体文库 插入片段 稳定性 |
| 英文关键词:Triticum aestivum Bacterial artificial chromosome library Insert segment Stability |
| 基金项目:国家现代农业产业技术体系项目（CARS 3 1）；石家庄市科技支撑计划项目（12149402A） 。 |
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| 中文摘要: |
| 为给小麦功能基因研究提供参考信息，以冬小麦栽培品种石麦15（Triticum aestivum L.）为试材，从暗培养7~10 d的黄化幼苗地上部提取高分子量基因组DNA，使用载体pCC1BAC的HindⅢ位点构建了六倍体小麦细菌人工染色体文库。该文库由1 000 000 个以上克隆组成，保存在1 020个混合池中，克隆插入片段平均大小为85 kb，空载率为2.5%；覆盖小麦基因组5倍以上；挑取7个克隆培养5 d 后，经HindⅢ完全酶切检测，其指纹图谱稳定一致。石麦15细菌人工染色体文库的构建为小麦基因克隆、调控序列克隆以及比较基因组学研究奠定了基础。 |
| 英文摘要: |
| A bacterial artificial chromosome library of hexaploid wheat was constructed using the winter wheat （Triticum aestivum L.）cultivar ‘Shimai 15’. Fresh shoot tissue from 7~ 10 day old seedlings was used to isolate HMW genomic DNA. The library was constructed using the HindⅢ site of pCC1BAC vector(HindⅢ). The whole library contained more than 1 000 000 BAC clones and preserved in 1020 pools. The average insert size of BAC clones is about 85 kb and the empty clones are about 2.5%. The library represents common wheat genome coverage of five fold. Seven clones picked randomly from this library showed no HindⅢ fingerprint changes after cultured 5 days，which indicates that the BAC clones are stable in the library. The library lays the foundation for gene cloning，regulatory sequence cloning and comparative genomics research of common wheat. |
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