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阳 霞,简俊涛,朱传杰,张兆萍,孙道杰,张玲丽.小麦抗病基因 Lr68Sr2 / Yr30的遗传特性分析[J].麦类作物学报,2014,34(2):151
小麦抗病基因 Lr68Sr2 / Yr30的遗传特性分析
Genetic Characteristic of Wheat Rust Resistance Genes Lr68 and Sr2 / Yr30
  
DOI:10.7606/j.issn.1009-1041.2014.02.02
中文关键词:  小麦  抗病基因  遗传特性  传递频率  农艺性状
英文关键词:Wheat  Disease resistance gene  Genetic characteristic  Gene transfer  Agronomic traits
基金项目:“十二五”农村领域国家科技计划项目(2012AA101105);美国唐仲英基金会项目;科技部支撑计划项目(K303021304)。
作者单位
阳 霞,简俊涛,朱传杰,张兆萍,孙道杰,张玲丽 (西北农林科技大学农学院陕西杨凌 712100) 
摘要点击次数: 1971
全文下载次数: 1346
中文摘要:
      为深入了解小麦抗病基因的遗传规律,以亲缘关系较远的小麦品种西农979和RL6077为亲本,构建其BC1F1和F2群体,用特异性标记csGS和WMS533分别对抗叶锈病基因 Lr68、抗秆锈病和条锈病基因 Sr2 / Yr30进行检测,同时进行农艺性状分析。结果表明,后代群体抗病基因分布广泛,F2群体抗病基因传递率(65.41%、74.53%、48.91%)较BC1F1(44.27%、55.11%、23.66%)高, Lr68基因在两群体中的传递频率均比理论值低,不符合一对显性基因的遗传规律, Sr2 / Yr30基因在BC1F1中的传递率比理论值高,在F2群体中正常,且后代群体中Sr2 / Yr30基因的分布较广。后代群体在有效分蘖数、株高、穗粒数、千粒重等农艺性状上与西农979无显著差异,有效小穗数与RL6077一致,茎部指标处于两亲本之间。总体来看,后代群体保留了西农979分蘖力较强、成穗率较高、穗型中等偏大、产量稳定等优良性状,且在分蘖数、成穗率、穗粒数上还有进一步提升的空间。
英文摘要:
      To deeply comprehend the gene heredity rule of wheat disease resistance and improve wheat agronomic characters, we used far kinship wheat cultivars as parents to estabish the F2 and BC1F1 descendant population. With the tag csGS and WMS533 to detected the Lr68 and Sr2/Yr30 gene, respectively, the correlation analysis of agronomic traits was carried out. The result showed that: the resistance genes were widely distributed in offspring population. The transfer rate of resistant genes in the F2 population (65.41%,74.53%,48.91%) extended higher than that in the BC1F1 population(44.27%,55.11%,23.66%). Sr2/Yr30 gene delivered normally, Lr68 gene transferred lower than expected. Among the offspring population, Sr2/Yr30 gene took wider distribution. On the agronomic traits, the effective tillers, plant height, grain numbers per ear and thousand seed weight of offspring population were consistent with Xinong 979, and effective spikelet number was consistent with RL6077, the stem index was between the two parents. Overall, offspring population inherited the strong tillering, high earing rate, medium to large panicle and good properties such as yield stability of Xinong 979, and there was further room to improve the tillering number, earing rate, and spike grain number. This study narrowed the selection scope of wheat breeding in the early generation, as it also reduced workload. It has important reference meaning in the breeding of varieties which may carry resistance genes as well as high yield.
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