| 李春莲，薛 芳，靳 凤，张芸芸，王中华，陈耀锋，胡小平.普通小麦DH群体条锈病抗性鉴定及抗病基因的分子检测[J].麦类作物学报,2013,33(6):1300 |
| 普通小麦DH群体条锈病抗性鉴定及抗病基因的分子检测 |
| Evaluation of Stripe Rust Resistance and Detection of Resistance Gene in a Common Wheat DH Population |
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| DOI：10.7606/j.issn.1009-1041.2013.06.039 |
| 中文关键词: 普通小麦 DH群体 条锈病 抗病基因 |
| 英文关键词:Common wheat DH population Stripe rust Resistant gene |
| 基金项目:国家“973”计划项目(2013CB127700)；国家自然科学基金项目（31271985）；国家公益性行业（农业）计划项目（200903035）。 |
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| 中文摘要: |
| 为了给小麦抗条锈病育种提供参考，对源自普通小麦杂交组合（西农1376×WT212）×花育888的DH群体进行了条锈病抗性鉴定及抗病基因的分子检测。结果表明，DH群体中对当前小麦条锈菌流行小种条中32（CYR32）具有苗期抗性的株系占50.7%，具有成株期抗病性的株系占51.8%。此外，利用对 Yr10、 Yr15和 Yr26基因有效的分子标记在群体中的检测结果表明，该DH群体中有141个株系（51.5%）含有 Yr10基因，但没有检测到 Yr26和 Yr15基因的存在；含有 Yr10基因的株系均为DH群体中对CYR32具有抗病性的株系，反应型为免疫~中抗（IT：0~2），表明 Yr10基因对我国当前条锈病流行小种具有良好的抗性。 |
| 英文摘要: |
| This study was to evaluate the stipe rust resistance of 274 DH wheat (Triticum aestivum L.) lines derived from the cross of (Xinong1376 × WT212) × Huayu888, and to detect the stripe rust resistant genes in the population by molecular markers. Currently epidemic race CYR32 of Puccinia striiformis f. sp. tritici was used to identify the seedling and adult-plant stripe rust resistance, and three stripe rust resistant genes, Yr10, Yr15, and Yr26 were detected by molecular markers, respectively. There were 50.7% of DH lines against CYR32 at seedling stage, and 51.8% at adult-plant stage. Yr10 gene was detected in 51.5% of DH lines, but Yr26 and Yr15 were not detected in the population. The infection type of the lines that harboring Yr10 were from immune to medium resistance (IT：0~2). |
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