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禾谷孢囊线虫致病型的鉴定对于抗性品种的选育和利用非常重要。传统的致病型鉴定工序繁杂，费工费时。本研究共筛选147条随机引物，找到3个燕麦孢囊线虫荥阳群体致病型相关的RAPD标记，其中引物S10扩增出1条780 bp的多态性片段，S43扩增出1条1 300 bp的多态性片段，S112扩增出1条875 bp的多态性片段。这3个RAPD标记只在燕麦孢囊线虫荥阳群体上出现，而在黄淮麦区其他禾谷孢囊线虫群体不同致病型上均没有扩增条带，说明这3个RAPD标记确实是燕麦孢囊线虫荥阳群体致病型相关的分子标记，为下一步将RAPD标记转化为SCAR标记提供了依据，可以用于燕麦孢囊线虫荥阳群体致病型的检测和鉴定。

英文摘要:

Wheat disease caused by cereal cyst nematode(CCN)， Heterodera avenae, has been the important disease in Henan province,and it is becoming the enormous threat to wheat production. Pathotype characterization is important for breeding and utilization of disease-resistant varieties. Traditional procedure of pathotype identification is time-consuming. In this research,RAPD technique was used to build the molecular marker realated to the pathotype of CCN. 147 random primers were screened and 3 random primers with RAPD method S10,S43,S112 were found relating to Xingyang population of CCN with Polymorphic bands of 780 bp,1300 bp,and 875 bp respectively. The results of PCR amplification with S10,S43,S112 in other CCN pathotype from the wheat region of huang-huai floodplain in China showed that the Polymorphic bands appeared only in Xingyang population of CCN. The three RAPD markers related to Xingyang population of CCN can be used to detect and identify the CCN pathotype of Xingyang population.

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