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为了解UV-B辐射对小麦光信号转导通路的影响，以冬小麦品种晋麦8号为材料，对不同UV-B剂量（10、12和14 kJ·m^-2·d^-1）辐射处理的小麦幼苗分别施用钙调蛋白（CaM）拮抗剂、G蛋白激活剂、Ca²⁺拮抗剂、丝氨酸/苏氨酸蛋白激酶拮抗剂，分析了光信号通路各组分的变化。结果表明，（1）CaM、G蛋白、Ca²⁺、丝氨酸/苏氨酸蛋白激酶和花色素苷参与了UV-B辐射诱导的小麦光信号通路的传导；（2）低剂量和高剂量的UV-B辐射对花色素苷含量的调控不是通过同一条信号通路；（3）UV-B辐射强度为10和12 kJ·m^-2·d^-1时，会诱导查尔酮合酶（CHS）基因的表达，而14 kJ·m^-2·d^-1UV-B辐射强度会抑制CHS基因的表达。说明UV-B辐射影响植物生理生化的信号通路因辐射剂量而异。

英文摘要:

In order to understand the effects of UV-B radiation on the signal transduction pathway of wheat, wheat seedlings of Jinmai 8 were treated with different doses (10 kJ·m^-2·d^-1, 12 kJ·m^-2·d^-1, 14 kJ·m^-2·d^-1) of UV-B radiation, then several antagonists such as CaM antagonists, G-protein activator, Ca²⁺ antagonists, serine/threonine protein kinase were added to analyze the changes in the components of light signal transduction. By fluorescence spectroscopy and northern blotting analysis, the results showed that CaM, G-proteins, Ca²⁺, a serine / threonine protein kinase, anthocyanin were involved in the transmission of light signaling pathway of UV-B radiation-induced wheat; Low doses and high doses of UV-B radiation induced different signaling pathway to product anthocyanin; 10 kJ·m^-2·d^-1 and 12 kJ·m^-2·d^-1 UV-B radiation induced the expression of chalcone synthase (CHS) gene, while 14 kJ·m^-2·d^-1 UV-B radiation inhibited its expression. Therefore, it was concluded that low doses and high doses of UV-B radiation resulted in two different signaling pathways for regulation of plant physiology and biochemistry.

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