In this study, two winter wheat cultivars (JD8 and JD11) were selected and designed with four levels of basic seedlings (210, 300, 390, and 480 seedlings per m) to investigate the effect of plant density on the size distribution of endosperm starch granule, which were detected using a laser diffraction particle size analyzer (LS13320, Beckman Coulter Co.). The results indicated that the size distribution of starch granule of wheat cultivar JD8 showed a bimodal curve under 390 seedlings per m condition, while in other three treatments revealed trimodal curves. Cultivar JD11 had a bimodal curve only under the lowest seedling level, while in other levels showed trimodal curves. Ratio of A type starch granules (diameter>10 μm), mean starch diameter, and medium starch diameter were enhanced with the increase of plant density in a reasonable range. In conclusion, plant density greatly affected the size distribution of starch granule in wheat endosperm. Within a wheat cultivar endosperm, there were either two types (A type:diameter>10μm and B type:diameter ≤10μm) or three types (A type:diameter >10 μm, B type:5 μm < diameter ≤10 μm, and C type:diameter ≤5 μm) starch granules. |