| 1B·1R translocation, subunits of high molecular weight glutenin, polyphenol oxidase (PPO) activity and yellow pigment content have major impacts on wheat processing quality, and slow rusting gene Lr34 locus is resistant to leaf rust, stripe rust, powdery mildew and stem rust. The accurate and rapid identification of these genes are important for the improvement of wheat quality and disease resistance. In this study, locus specific molecular markers for high molecular weight glutenin subunits of Dx5 and By8, 1B·1R translocation, PPO, PSY gene and Lr34 were used to characterize 169 wheat varieties from different regions of Heilongjiang Province to know the distribution of these genes in wheat varieties of Heilongjiang province, and to verify the reliability and accuracy of those molecular markers, which could provide materials and methods for molecular marker assisted breeding in wheat with good quality and disease resistance. The results showed that the frequencies of Dx5, By8, 1B·1R translocation, Ppo A1b, Psy A1b and Lr34 were 43.2%, 30.8%, 4.7%, 33.1%, 33.7% and 24.9%, respectively; only two lines were detected without 1B·1R translocation and with all the loci of Dx5, By8, Ppo A1b, Psy A1b and Lr34 locus. The gene specific markers used in the present study were repeatable and highly accurate to be effectively used in marker assisted selection for breeding wheat varieties with good quality and disease resistance. |
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