| Leaf rust of wheat, caused by Puccinia triticina, is the most common rust disease worldwide. Wheat leaf rust resistance gene Lr9, derived from wheat Aegilops umbellulata chromosome translocation of T6BS·6BL 6U#1L, was highly resistant to prevailing P. triticina isolates THT and PHT in China. An American common wheat variety Arthur 71 was used as donor of Lr9 to make cross with a highly susceptible common wheat line Xuezao for genetic and bulked segregated analyses using F2 population and F2:3 families. Six SSR markers, Xwmc179, Xbarc146, Xbarc198, Xcfa2110, Xbarc24, and Xbarc178, one EST STS marker BE443156, and one EST SSR marker XMAG799, were found to be linked to Lr9. Xwmc179, Xbarc146, BE443156 and XMAG799 were dominant markers, while Xbarc198, Xcfa2110, Xbarc24 and Xbarc178 were co dominant markers. Lr9 was co segregated with EST STS marker BE443156, was in 3.4 cM distal to Xbarc178 and assigned to the distal bin of chromosome 6BL. STS marker J13/1+2 and SCAR marker SCS5550 were proved to be co segregated with Lr9 and could be used as powerful tool in marker assisted selection of Lr9. The SSR markers identified in this study could be used as markers for background selection.By applying a “rolling cycle accelerated backcrossing” approach, we developed a series of leaf rust resistance lines containing Lr9 with improved agronomic traits. |
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