| 郭 杰，庄丽芳，亓增军.百萨偃麦草ThbGSK和 ThbHKT1基因的同源克隆与表达分析[J].麦类作物学报,2013,33(2):209 |
| 百萨偃麦草ThbGSK和 ThbHKT1基因的同源克隆与表达分析 |
| Cloning and Expression Analysis of Glycogen Synthase Kinase gene ThbGSK and High affinity K+ Transporter gene ThbHKT1 of Thinopyrum bessarabicum |
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| DOI：10.7606/j.issn.1009-1041.2013.02.01 |
| 中文关键词: 百萨偃麦草 GSK基因 HKT1基因 同源克隆 基因表达 |
| 英文关键词:Thinopyrum bessarabicum GSK gene HKT1 gene Cloning Gene Expression |
| 基金项目:国家自然科学基金项目(31170302)。 |
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| 中文摘要: |
| 百萨偃麦草（Thinopyrum bessarabicum Lve, 2n=2x=14, JJ）是小麦改良的重要亲缘物种。为了解GSK和 HKT1基因在百萨偃麦草耐盐胁迫中的作用，本研究采用同源克隆的方法从百萨偃麦草中扩增获得了糖原合成酶激酶基因ThbGSK及高亲和性钾离子转运蛋白基因 ThbHKT1的全长cDNA，ThbGSK基因全长1 233 bp，与水稻、短柄草、小麦等物种的氨基酸一致性为93.92%~99.02%，表明GSK基因在这些物种间具有较高的保守性；聚类分析表明，ThbGSK与小麦TaGSK关系最近。 ThbHKT1基因全长1 602 bp，与水稻、小麦、短柄草、大麦等物种的氨基酸一致性为66.17%~92.87%，说明 HKT1基因在这些物种间变异较大；聚类分析表明， ThbHKT1与大麦 HvHKT1关系最近。RT PCR和荧光定量PCR分析表明，经250 mmol·L-1 NaCl胁迫处理后，GSK基因在百萨偃麦草和中国春诱导0~24 h的叶片中表现为组成型表达，而 HKT1基因在百萨偃麦草和中国春中均表现为诱导后上调表达。 |
| 英文摘要: |
| Thinopyrum bessarabicum Lve (2n = 2x = 14, JJ) represents an important genetic resource for wheat improvement. In this study, two full length orthologous genes of glycogen synthase kinase (GSK) designated ThbGSK and high affinity K+ transporters (HKT) designated ThbHKT1 were cloned from Th. bessarabicum leaf cDNA. In comparison with that of rice, Brachypondium distachyon and wheat, the identity of amino acid of the gene varied from 93.92%~92.02%, indicating high conservation of this gene in these species. Cluster analysis showed a closer relationship between ThbGSK and TaGSK1. In comparison with that of rice, wheat, Brachypondium distachyon and barley, the identity of amino acid of the gene ThbHKT1 varied from 66.17%~92.87%, indicating variation of this gene in these species. Cluster analysis showed a closer relationship between ThbGSK and HvGSK1. Stressed with 250 mmol·L-1 NaCl for 0~24 h, GSK showed constitutive expression patterns both in Th.bessarabicum and Chinese Spring while HKT1 showed up regulated after semi quantitative and quantitative analysis. |
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