| 蒋 明, 尹龙飞, 张志仙, 余沁欣, 罗礼礼.二穗短柄草WRKY基因家族成员的鉴定和分析[J].麦类作物学报,2012,32(6):1013 |
| 二穗短柄草WRKY基因家族成员的鉴定和分析 |
| Genome-wide Identification and Analysis of WRKY Gene Family Members from Brachypodium distachyon |
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| DOI：10.7606/j.issn.1009-1041.2012.06.001 |
| 中文关键词: 二穗短柄草 WRKY 基因家族 鉴定 |
| 英文关键词:Brachypodium distachyon WRKY Gene family Identification |
| 基金项目:浙江省公益性技术应用研究计划项目（2012C32011）。 |
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| 中文摘要: |
| WRKY转录因子以基因家族形式存在，在植物生长、发育和逆境反应中起着重要作用。二穗短柄草是大麦、小麦和禾本科牧草的模式植物，为了给基因表达和功能鉴定奠定基础，本研究利用生物信息学方法鉴定和分析其WRKY转录因子。结果表明，二穗短柄草有76个WRKY基因，它们具有0~6个内含子，编码区全长396~2 847 bp，基因分布在5条染色体上；进化分析结果表明，二穗短柄草WRKY在系统发生树上聚为3组，分属于I、II和III类转录因子；核心序列除WRKYGQK外，还有WKKYGQK、WRKYGKK、WRKYGEK、WRKYGQT和WKKYGPK等多种变异类型；锌指模体也存在多种变异，它们是C-X4-C-X21-H-X1-H、C-X3-C-X22-H-X1-H、C-X7-C-X28-H-X1-C、C-X7-C-X24-H-X1-C、C-X7-C-X26-H-X1-C、C-X6-C-X24-H-X1-C、C-X7-C-X33-H-X1-C、C-X6-C-X32-H-X1-C和C-X7-C-X32-H-X1-C。 |
| 英文摘要: |
| WRKY transcription factors belong to a gene family, and play important roles in plant growth, development as well as stress responses. Brachypodium distachyonacts as a model plant for Hordeum vulgare, Triticum aestivum and Gramineae grasses. To provide a basis for gene expression and functional characterization of genes, bioinformatic techniques were performed to identify WRKY transcription factors. Results indicated that there were 76 WRKY genes in B.distachyon with 0~6 introns. The coding sequences were 396~2 847 bp in length, and the genes distributed in 5 different chromosomes. Phylogenetic analysis revealed that the WRKYs were clustered into 3 groups and they belonged to I, II and III transcription factors, respectively. Besides WRKYGQK, several core sequence variants as WKKYGQK, WRKYGKK, WRKYGEK, WRKYGQT and WKKYGPK were identified. Mutations in zinc finger motifs were also observed, the variants included C-X4-C-X21-H-X1-H, C-X3-C-X22-H-X1-H, C-X7-C-X28-H-X1-C, C-X7-C-X24-H-X1-C, C-X7-C-X26-H-X1-C, C-X6-C-X24-H-X1-C, C-X7-C-X33-H-X1-C, C-X6-C-X32-H-X1-C and C-X7-C-X32-H-X1-C. |
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