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周国雁,伍少云,李文春.云南铁壳麦抗条锈病基因的SSR分子初步检测[J].麦类作物学报,2012,32(5):870
云南铁壳麦抗条锈病基因的SSR分子初步检测
Primary SSR Detection of Stripe Rust Resistance Gene of Yun Hulled Wheat
  
DOI:10.7606/j.issn.1009-1041.2012.05.011
中文关键词:  云南小麦,SSR,分子检测,抗条锈病基因
英文关键词:Triticum aestivum ssp.yunnanense King  SSR  Molecular detection  Stripe rust resistance gene
基金项目:云南省重点基金项目(2008C010);云南省农作物资源生物技术创新团队项目;云南省农业科学院资助项目。
作者单位
周国雁,伍少云,李文春 (1.云南省农业科学院生物技术与种质资源研究所/云南省农业生物技术重点实验室/农业部西南作物基因资源与种质创制重点实验室云南昆明 650205 2.云南中医学院云南昆明 655000) 
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中文摘要:
      为探讨云南小麦亚种(Triticum aestivum ssp yunnanense King,俗称铁壳麦)不同品种可能携带的已知和未知的抗条锈病基因,用38对与51个已知小麦抗条锈病基因连锁的SSR引物,对37份云南铁壳麦进行了SSR分子检测。结果显示,云南铁壳麦携带有丰富的与已知抗条锈病基因 Yr9 Yr10 Yr29 Yr41 YrTp1 YrSM139、YrSP、YrGn6 Yrvav B1 YrSyria YrXu YrM8003YrCD等连锁的SSR位点,其中24份具有与中国春相同或类似、且与Yr18连锁的 Xgwm295标记。用8对引物 Xgwm582、Xwmc477、Xwmc364 、Xgwm429、Xwmc702、Xwmc438、Xwmc810 Xgdm116检测到部分云南铁壳麦携带来自黑麦的 Yr9 YrM8003、长穗偃麦草的 YrTp1 YrTp2、华山新麦草的YrHy和中间偃麦草的 Yr88375YrZhong22抗条锈病基因的片段,并可能含有抗条锈病新基因,应重视其发掘与定位研究;许多云南铁壳麦含有高度慢条锈病的 Yr18基因,应在云南小麦新品种选育中高度重视和加以利用。
英文摘要:
      In order to study the diverse varieties of the Triticum aestivum ssp. yunnanense King,also called Yunnan hulled wheat, possibly carried the known and unknown stripe rust resistance genes, this study has given a preliminary molecular inspection for 37 Yunnan hulled wheat by using 38 SSR primers which were linked to 51 known stripe rust resistance genes. The results indicated that it had the rich loci linked to the known stripe rust resistance genes including Yr9, Yr10, Yr29, Yr41, YrTp1, YrSM139, YrSP, YrGn6, Yrvav B1, YrSyria, YrXu, YrM8003 and YrCD etc.; There were 24 accessions which accounted for about 65% of all test materials contained identical or similar maker to the Chinese Spring and linked to the Yr18 by Xgwm295 primer. The fragments of stripe rust resistance related genes with Yr9 and YrM8003 from Rye, YrTp1 and YrTp2 from Thinopyrum intermedium, YrHy from Psathyrostachys huashanica and Yr88375 and YrZhong22 from Thinopyrum ponticum were detected in Yunnan hulled wheat by 8 SSR primers of Xgwm582, Xwmc477, Xwmc364, Xgwm429, Xwmc702, Xwmc438, Xwmc810 and Xgdm116.This results indicated that Yunnan hulled wheat may contain new stripe rust resistance genes,and we should pay attention to their study on stripe rust resistance gene discovery and location. Many Yunnan hulled wheat were characterized to have slow stripe rust resistance gene Yr18, these wheat materials should were regarded as an important materials and utilized in Yunnan wheat breeding project.
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