| Barley is the fourth most important cereal crop worldwide, and is an ideal research model for Triticeae crops. To facilitate functional studies on Triticeae crops, Ethyl methane sulfonate (EMS) based mutagenesis was performed on an elite barley cultivar Tamalpais, and a mutant population harboring 10 389 M2individuals was developed. Under greenhouse condition, 6.21% of the tested seedlings displayed variations in leaf chlorophylls. In field, the population showed abundant phenotypic variations, such as those in creeping growth habit, number of tillers, plant height, flowering time, leaf color, leaf shape, leaf stripe, leaf spot, spike characteristics, and sexual reproduction. In particular, mutation rates for creeping growth habit, number of tillers, plant height, leaf color, leaf stripe, and leaf spot were 0.11%, 6.03%, 0.13%, 2.5%, 0.18%, and 0.17%, respectively. Sampling survey revealed that the population was low on embryo lethality. About 9% of surveyed M2individuals had rates of embryo lethality over 50%. Mutants of the COI1 gene, encoding a crucial receptor in the jasmonate acid (JA) signaling pathway, were identified using the TILLING technique. The mutation frequency is about 1 point mutation in every 673 kb region. Taken together, the current population is enriched for variations on many agronomically important traits and is suitable for TILLING screening.The Tamalpais population will play roles for functional studies in Triticeae crops. |
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