齐冰洁,张智勇,赵利梅,刘景辉,张向前.燕麦种质资源遗传多样性的AFLP分析[J].麦类作物学报,2012,32(4):648 |
燕麦种质资源遗传多样性的AFLP分析 |
Genetic Diversity of Oat Germplasm Revealed by AFLP Analysis |
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DOI:10.7606/j.issn.1009-1041.2012.04.009 |
中文关键词: 燕麦(Avena sativa L.) 种质资源 扩增片段长度多态性(AFLP) 遗传多样性 |
英文关键词:Oat Germplasm resource AFLP Genetic diversity |
基金项目:内蒙古科技计划项目(20090708,20100704);内蒙古自然科学基金项目(2009MS0306,2010ZD07);内蒙古农业大学科技创新团队建设计划项目(NDTD2010 8)。 |
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中文摘要: |
为给燕麦种质资源的收集保护和育种研究提供依据,利用扩增片段长度多态性(AFLP)技术对74份燕麦种质资源的遗传多样性进行了分析。结果表明,10对AFLP引物共产生784个扩增位点,多态性位点比率达96.91%;供试燕麦种质的平均Nei′s指数为0.382,平均Shannon′s信息指数为0.573,各供试材料的AFLP多态性丰富,表现出较高的遗传多样性,利用AFLP标记评价燕麦种质的遗传多样性具有较高的检测效率。对AFLP数据进行聚类分析,构建了供试燕麦种质的UPGMA系统进化树,74份燕麦种质可以聚为五大种质群,聚类结果与地理来源及皮裸性有一定的相关性。 |
英文摘要: |
In order to provide scientific basis for oat germplasm resource collection,protection and breeding,the genetic diversity of 74 oat germplasm were analyzed by AFLP. The results showed that the average Nei's index (H) and Shannon's information index was 0.382 and 0.573,respectively. The test materials had rich AFLP polymorphism and high genetic diversity,therefore,to evaluate the genetic diversity of oat germplasm by AFLP had higher efficiency. 74 oat germplasm resources were divided into five groups by cluster analysis,which seemed related to the geographical origins and naked or covered oat. The UPGMA systematic tree was obtained by cluster analysis. |
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