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作者	单位
薛晓丽，张晓军，李欣，张丛卓，詹海仙，畅志坚	（1.山西大学生物工程学院，山西太原 030006； 2.山西省农业科学院作物科学研究所，山西太原 030031）

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中文摘要:

为了给小麦品质育种提供有益材料和信息，利用SDS PAGE技术对311份新育成的源于偃麦草的高代渗入系的HMW GS组成进行了分析。结果表明，在参试材料中共检测到16种不同的亚基类型：在Glu A1位点有Null、2^*和1亚基等3个等位变异类型，以优质亚基1和2^*为主要类型，占测试材料的71.1%；Glu B1位点有7、7+8、7+9、6+8、13+16、13+19、14+15、17+18等8个等位变异类型，以17+18为主要类型，其次为7。优质亚基13+16、14+15和17+18的出现频率共计53.4%；Glu D1位点有2+12、5+10、2+10、2+12、5+12亚基5个等位变异类型，以2+12为主要类型，占53.7%，其次为5+10，占38.9%。同时发现共有32种亚基组合，以“2^*、17+18、2+12”的出现频率最高，为13.2%；其次为“1、17+18、5+10”，占10.6%。品质得分为8~10分的材料有164份，占52.73%。由此可见，在这些小麦中间偃麦草高代渗入系中存在丰富的高分子量谷蛋白亚基变异，可能对小麦品质改良具有重要的应用价值。

英文摘要:

To provide useful materials and information for wheat breeding, we analyzed the composition and allelic variation of HMW glutenin subunits by SDS PAGE in a total of 311 wheat alien introgression lines from Thinopyrum intermedium. Three allelic variants of Null, 2^* and 1 were detected at Glu A1 loci, with 2^* and 1as the major types (71.1% of total materials). Eight alleles, including the high quality scored subunits of 14+15, 17+18 and 13+16 with a total frequency of 53.4%, were detected at the Glu B1 loci, with 7+8 as the major type, followed by 7 type. While five alleles for the Glu D1 loci were detected, among which 2+12 was the major type with a frequency of 53.7% and then followed by 5+10 with a frequency of 38.9%. Considering the three loci, thirty two combinations were found among all the evaluated lines, in which combination of 2^*, 17+18, 2+12 had the highest frequency of 13.2%, followed by the combination of 1, 17+18 and 5+10 with the frequency of 10.6%. The materials with high quality score, between 8 to 10 accounted for 52.73% of total. The wide variation among those lines suggested that they were useful to enhance the quality of bread wheat.

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