| 张跃强，李剑峰，王 重，樊哲儒，王 浩，张宏芝.干旱胁迫下小麦幼苗基因表达谱的cDNA AFLP分析[J].麦类作物学报,2012,32(2):240 |
| 干旱胁迫下小麦幼苗基因表达谱的cDNA AFLP分析 |
| Gene Expression Profile of Wheat Seedling Response to Drought Stress by cDNA AFLP |
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| DOI：10.7606/j.issn.1009-1041.2012.02.009 |
| 中文关键词: 小麦 干旱胁迫 基因表达 |
| 英文关键词:Spring wheat Drought stress Gene expression |
| 基金项目:新疆维吾尔自治区高技术研究发展项目(201011109)；国际农业磋商组织（CGIAR）挑战计划项目(G7010.02.01 5)；新疆农作物生物技术重点实验室开放课题项目(XJYS0302 2007 01)。 |
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| 中文摘要: |
| 为了分离和识别小麦抗旱相关基因，给小麦抗旱节水育种提供依据，以既抗旱又具备高水分利用效率的新疆春小麦主栽品种新春6号为材料，采用cDNA AFLP技术，分析了小麦在两叶一心期干旱胁迫条件下的诱导表达基因。通过253对引物组合的筛选，共得到748个干旱胁迫特异上调表达的转录衍生片段（TDF）。对其中22条片段进行克隆、测序、Blastx比对和功能分类分析的结果表明，有18个TDFs所推导的蛋白质序列与NCBI已有序列同源，功能涉及逆境胁迫反应、发育、信号转导、抗病蛋白、跨膜转运、能量代谢等方面；有4个TDFs在NCBI找到同源序列，但功能未知。 |
| 英文摘要: |
| Drought is one of the most adverse environmental factors affecting wheat yield and quality. Under drought stress, target genes in wheat could to be activated and expressed to produce drought induced proteins, enabling plants to actively adapt to drought, and to maintain survival and yield formation. So, studying on drought induced proteins and the related genes is essential for future genetic engineering and crop breeding of drought resisting and water saving. The objective of this research is to analyze the gene expression profile of wheat under drought stress, and to detect the fragments related to drought resistant gene expression. Genes induced by drought stress in the seedling of Xinjiang spring wheat cultivar Xinchun 6 at 2 leaf stage were analyzed by cDNA AFLP techniques. A total of 253 primer combinations were screened, about 3500 positive cDNA fragments were detected. Of these, 748 upregulated differentially expressed transcript derived fragments (TDFs) were identified, and 22 TDFs were sequenced and analyzed. BLASTx analysis and functional annotation revealed that 18 TDFs had homologous with genes of known function in GenBank database, and 4 TDFs were similar to some unknown function genes. The TDFs with known function protein homology conferred functions of stress response, development, signal transduction, disease resistance protein, transmembrane, and energy metabolism, etc. These results accumulated information for further elucidation of molecular mechanism of wheat cultivar Xinchun 6 to drought stress, and provide some candidate genes for crop genetic engineering of drought resisting and water saving. |
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