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为了拓宽农杆菌介导的小麦遗传转化的受体基因型，同时改良小麦品种的抗蚜特性，以甘肃省主推春小麦品种陇春22和优良品系9614的幼胚为转基因受体材料，以预培养4 d的幼胚愈伤组织为受体，以含有半夏凝集素基因的C58c1农杆菌菌株为供体，将重组质粒pBIpta导入小麦，建立了农杆菌介导的小麦遗传转化体系。经G418 25 mg·L^-1抗性筛选、PCR检测共获得转基因植株7株，其中9614的4株，陇春22的3株；采用荧光定量PCR分析得出转基因植株中单拷贝的有1株，2个拷贝的1株，3个拷贝和4个拷贝的各有2株，1株没有得到扩增。对整合有2个拷贝半夏凝集素基因的陇春22 20转基因小麦的T₁代植株进行了PCR检测，其中有50% 的植株扩增出目的基因，50%没有扩增出目的基因，初步说明T₁代植株中外源基因得到了遗传。

英文摘要:

In this study, the genetic transformation system of wheat mediated by Agrobacterium tumefacien was set up by using immature embryos of spring wheat cultivar 9614 and Longchun 22 as explants. The immature embryos after 4 days preculture were transformed with pta gene using Agrobacterium tumefaciens C58c1. Seven transgenic plants (4 come from 9614 and 3 come from Longchun 22) were obtained after screening by G418 resistance and PCR analysis. Real time PCR analysis showed that the transgenic wheat plant with 1 and 2 copies of pta gene was one for each and with 3 and 4 copies of pta gene was 2 for each, and there is no pta gene amplification in one transgenic plant of 9614-17. 50% T₁ progeny of transgenic wheat longchun22-20 was integrated with exogenous pta gene by PCR analysis, and the other 50% wasn't. This was the preliminary evidence that pta gene had been inherited to the T₁ progeny of transgenic wheat.

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