| 张丽琴， 刘春雷， 杨 雪， 王世杰，史丽红 ，王 曼.黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析[J].麦类作物学报,2012,32(1):79 |
| 黄淮麦区小麦新品种(系)高分子量谷蛋白亚基多态性分析 |
| Polymorphism Analysis of HMW GS in New Wheat Varieties in Huang huai Wheat Region of China |
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| DOI：10.7606/j.issn.1009-1041.2012.01.14 |
| 中文关键词: 黄淮麦区 小麦 高分子量谷蛋白亚基 多态性 品质 |
| 英文关键词:Huang huai wheat region Wheat HMW GS Polymorphism Quality |
| 基金项目:国家自然科学基金项目(31071413); 河南省基础与前沿技术研究计划项目(082300430010); 河南教育学院青年基金项目(20100104); 河南教育学院生态学重点学科资助项目。 |
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| 中文摘要: |
| 为了解黄淮麦区小麦新品种(系)的品质状况,用SDS PAGE方法对227份材料的HMW GS组成进行了分析, 共检测到10种等位基因变异和18种亚基组合, 其中, 各位点的主要亚基有:Glu A1位点的1(61.67%),Glu B1位点的7+9(62.11%)和7+8(21.59%), Glu D1位点的2+12(53.30%)和5+10(40.97%)；主要的亚基组合为“1, 7+9, 2+12”（21.59%)和“null, 7+9, 2+12”（19.38%)。研究表明，黄淮麦区小麦HMW GS等位变异和亚基组合的多态性较为丰富, 但分布严重不均, 仍需引进含有2*、13+16和17+18等亚基的材料; 优质亚基5+10和14+15的比例有所提高, 但优质亚基组合的比例仍然很低, 今后该地区小麦品质育种中应加强优质亚基组合的选择。 |
| 英文摘要: |
| In order to investigate the quality of new wheat varieties in Huang huai wheat region, HMW GS compositions of 227 materials were analyzed by SDS PAGE. 10 HMW GS alleles and 18 subunits combinations were detected, i.e, subunits 1 (61.67%) at Glu A1loci, 7+9 (62.11%) and 7+8 (21.59%) at Glu B1loci, 2+12 (53.30%) and 5+10 (40.97%) at Glu D1loci were the major subunits, and subunit combinations “1, 7+9, 2+12”（ 21.59%） and “null, 7+9, 2+12”（19.38%） were the major subunit combinations. The result showed that there were high polymorphism in alleles of HMW GS and subunit compositions, but the frequencies of different alleles were not balanced, and the breeding materials which hold subunits 2*, 13+16 and 17+18 should be introduced. Quality subunits 5+10 and 14+15 showed higher frequencies than before, but quality combinations showed low frequencies so selection of quality subunit combinations should be strengthened in this area in future. |
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