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作者	单位
谭彩霞，封超年，郭文善，朱新开，李春燕，彭永欣	(1.扬州大学江苏省作物遗传生理重点实验室，江苏扬州 225009；2.金陵科技学院，江苏南京 211169)

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中文摘要:

为探寻不同专用类型小麦籽粒品质调控的途径，以不同专用类型小麦品种为材料，比较了其籽粒淀粉合成酶基因表达、淀粉合成酶活性以及淀粉积累速率动态特征，并分析了三者之间的相关性。结果表明，整个灌浆期间，籽粒淀粉合成酶基因(AGPase1、GBSSI、SSSIII、SBEI)相对表达量、淀粉合成酶(AGPase、GBSS、SSS、SBE)活性及直、支链淀粉积累速率均表现为强筋小麦中优9507>中筋小麦淮麦18>弱筋小麦宁麦9号>糯小麦Wx11。相关分析表明，AGPase1、SSSIII、SBEI基因的相对表达量最大值与AGPase、SSS、SBE活性峰值均呈极显著正相关；GBSSI基因相对表达量最大值与GBSS活性峰值相关不显著；4种淀粉合成酶基因相对表达量最大值均与籽粒直、支链淀粉及总淀粉积累速率峰值呈极显著正相关；4种淀粉合成酶活性与籽粒直、支链淀粉及总淀粉积累速率均呈极显著正相关。

英文摘要:

Wheat cultivars of different gluten types were used for investigating the changes of starch synthase gene expression, starch synthase activity and starch accumulating rate. Results showed that, 1) During the entire filling of wheat the expression levels of starch synthase genes(AGPase1，GBSSI，SSSIII，SBEI), the activities of starch synthases(AGPase，GBSS，SSS，SBE) and amylase, amylopectin, and total starch accumulating rates were recorded in a descending order of Zhongyou 9507 (a strong gluten cultivar), Huaimai 18 (a medium gluten cultivar), Ningmai 9 (a weak gluten cultivar), and Wx11 (a Waxy variety). 2) The relationship between the maximum expression levels of AGPase1，SSSIII，SBEI and the peak activities of AGPase，SSS，SBE was significantly correlated at 0.01 levels, while there were no significant correlations between the maximum expression levels of GBSSI and the peak activities of GBSS. 3)The maximum expression levels of the four starch synthase genes were highly significantly correlated with the peak of amylase, amylopectin, and total starch accumulating rates. 4) There were highly significant correlations between the four starch synthase activities and amylase, amylopectin, and total starch accumulating rates.

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