| 杨 宏，邓光兵，龙 海，潘志芬，唐 建，彭云良，徐世昌，余懋群.普通小麦品种科成麦2号抗条锈病基因鉴定及分子作图[J].麦类作物学报,2011,31(5):978 |
| 普通小麦品种科成麦2号抗条锈病基因鉴定及分子作图 |
| Identification and Genetic Mapping of a Stripe Rust Resistance Gene in Wheat Cultivar “Kechengmai 2” |
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| DOI：10.7606/j.issn.1009-1041.2011.05.031 |
| 中文关键词: 小麦 条锈病抗性基因 SSR标记 遗传分析 |
| 英文关键词:Wheat Stripe rust resistance gene SSR marker Genetic analysis |
| 基金项目:中国科学院知识创新工程重要方向项目(KSCX3 EW N 02 2)；四川省育种攻关项目。 |
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| 中文摘要: |
| 普通小麦品种科成麦2号(咸阳大穗/E10//多花1号/3/贵农20/4/绵阳26)，对我国目前流行的小麦条锈菌生理小种条中32和水源11 4表现免疫或近免疫，而科成麦2号的近等基因系CD1438对条中32和水源11 4高感。为了给小麦抗条锈病育种提供参考依据，对科成麦2号/CD1438杂交组合的F1材料以及F2、F3群体进行了抗病性鉴定与遗传分析，结果表明，科成麦2号对条中32的抗性受细胞核内的显性单基因控制，暂命名为YrKC2。利用集群分离分析法(BSA)和简单重复序列(SSR)分子标记分析, 发现了7 个与YrKC2连锁的SSR标记并构建连锁标记遗传图谱, 其中Xcfd65/Xgwm11紧邻YrKC2，遗传距离为1.7 cM；Xgwm18、Xbarc187/Xwmc406、Xwmc419、Xwmc216依次排列，与 YrKC2的遗传距离分别为2.5、3.3、6.0、9.2 cM。根据SSR分子标记的遗传图谱，将YrKC2定位在小麦的1B染色体短臂上。通过系谱分析和分子标记分析，推测YrKC2可能来源于小麦品种贵农20。基因等位性鉴定表明，YrKC2可能与Yr26、Yr24和YrCH42互为等位基因。 |
| 英文摘要: |
| Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat (Triticum aestivum L.) worldwide. To explore resistant materials, identify resistance genes and construct genetic maps of resistance genes are important work of wheat breeding. Wheat cultivar “Kechengmai 2” was highly resistant to predominant Chinese PST race CRY32 and Shuiyuan11 4. A F2 population of 246 individuals was established by crossing Wheat cultivar “Kechengmai 2” with its sibling susceptible line CD1438. Genetic analysis indicated that the resistance of “Kechengmai 2” was controlled by a single dominant gene, temporarily designated YrKC2. Seven SSR markers were found to be linked with YrKC2 in the alignment of YrKC2 Xcfd65/Xgwm11 Xgwm18 Xbarc187/Xwmc406 Xwmc419 Xwmc216. Xcfd65 and Xgwm11were 1.7 cM distal of YrCD, Xgwm18, Xbarc187, Xwmc406, Xwmc419, Xwmc216were linked to YrCD with 2.5, 3.3 cM, 3.3 cM, 6.0 cM and 9.2 cM, respectively. YrKC2was located on the short arm of 1B chromosome of wheat according to the locations of the seven markers. Pedigree and SSR analysis indicated that YrKC2 might originate from wheat cv “Guinong 20”. An allelic test between “Kechengmai 2” and Chinese cultivar “Chuanmai 42” was performed and the relationship of YrKC2, YrCH42, Yr24and Yr26 was also discussed. |
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