李 楠, 宋 勇,陈 敏,郭伟伟,张文会.紫外线照射促进冬小麦种子萌发的研究[J].麦类作物学报,2011,31(5):935 |
紫外线照射促进冬小麦种子萌发的研究 |
Ultraviolet Irradiation Promoted Seeds Germination of Winter Wheat |
|
DOI:10.7606/j.issn.1009-1041.2011.05.023 |
中文关键词: 小麦种子 紫外线照射 发芽率 淀粉酶活力 |
英文关键词:Wheat seed Ultraviolet radiation Germination rate α amylase activity |
基金项目:国家自然科学基金项目(30771156)。 |
|
摘要点击次数: 1620 |
全文下载次数: 1154 |
中文摘要: |
为了解紫外线照射对种子萌发的影响,以冬小麦品系聊大2号为材料,采用不同波长的紫外线分别照射休眠的干种子及吸水萌动(浸种3 h)的种子,然后在自然光和黑暗条件下进行发芽实验,比较了不同时间段种子萌发的动态变化及萌发过程中α 淀粉酶活力的变化。结果表明,不同剂量的紫外线照射(UVB 85 μW·cm-2照射3 h;UVC 420 μW·cm-2照射1 h;UVC处理1 h附加照射6 h UVA或1 h UVB)对干种子和吸水萌动种子萌发均有促进作用,发芽较自然光下(CK)及黑暗下快,相关时段发芽率均高于CK,但不影响最终发芽率,只是缩短了种子萌发的时间。UV处理(UVB 85 μW·cm-2照射3 h;UVC 420 μW·cm-2照射1 h)的干种子及吸水萌动种子的α 淀粉酶活力在萌发前随时间的推移呈快速增加的趋势,与对照相比可提高2.5%~68.7%,发芽后α 淀粉酶活力下降,但仍高于CK,且UVB与UVC处理种子的α 淀粉酶活力变化趋势相同。说明不同波段的紫外线照射对种子具有相同的生物学效应。 |
英文摘要: |
To study the effects of ultraviolet radiation on seeds germination of winter wheat, we investigated the germination rate and α amylase activity of the dry seeds and water soaked seeds in the conditions of different wavelengths of ultraviolet radiation treatments. The results indicated that different duration and doses of UV radiation(UVB, 85 μW·cm-2, 3 h; UVC, 420 μW·cm-2, 1 h; UVC 1h+UVA 6 h; UVC 1h+UVB1h) promoted the germination rate of dry seeds and germinating seeds, the germination rate increased by 2.5%~269.2% compared with control, but didn't affect the final germination rate, only shorten the time of seed germination .In addition, the α amylase activity of the dry seeds and water absorbent seeds treated with UV radiation(UVB 85 μW·cm-2 3 h; UVC 420 μW·cm-2 1 h) increased rapidly by 2.5%~68.7% before germination compared with the control. After germination, the activity of α amylase decreased, but also higher than the control. Furthermore, UVB、UVC treatment have the same effects on changes of α amylase activity. Therefore, different wavelengths of ultraviolet radiation on seeds have the same biological effects. |
查看全文 查看/发表评论 下载PDF阅读器 |
关闭 |