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梁 强,王晓龙,张晓科,张 晶,张同兴,冯 毅,王 瑞,王宏礼.陕西小麦 Glu A3 Glu B3位点等位变异的检测和分析[J].麦类作物学报,2011,31(5):859
陕西小麦 Glu A3 Glu B3位点等位变异的检测和分析
Idetection and Analysis of Allelic Variations at Glu A3and Glu B3Loci in Shaanxi Wheats
  
DOI:10.7606/j.issn.1009-1041.2011.05.011
中文关键词:  小麦  LMW GS  STS标记   Glu A3   Glu B3
英文关键词:Triticum aestivum L.  LMW GS  STS markers   Glu A3   Glu B3
基金项目:“948”项目(2006 G2);小麦产业技术体系建设专项(nycytx 03);西北农林科技大学唐仲英育种基金项目。
作者单位
梁 强,王晓龙,张晓科,张 晶,张同兴,冯 毅,王 瑞,王宏礼 (1.西北农林科技大学农学院/国家小麦改良中心杨凌分中心陕西杨凌712100
2.陕西省岐山县农技中心陕西岐山722400) 
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中文摘要:
      低分子量谷蛋白亚基(LMW GS)与小麦品质密切相关。为了给陕西小麦的品质改良提供参考依据,采用STS分子标记,检测了175份陕西小麦品种(系) Glu A3 Glu B3位点的等位变异组成。结果表明,陕西小麦 Glu A3位点存在4种等位变异,即 Glu A3a Glu A3b Glu A3c Glu A3d,分别占12.6%、1.7%、58.3%和27.4%; Glu B3位点存在8种等位变异,即 Glu B3a Glu B3b Glu B3d Glu B3e Glu B3f Glu B3g Glu B3i Glu B3j,分别占4.6%、2.9%、45.7%、0.6%、2.9%、8.5%、4.0%和30.8%。在陕西不同地区小麦之间,两个位点等位变异的种类、组合及其分布比例存在差异,这可能与地区间不同的自然地理环境、饮食习惯、育种目标及亲本选择有关。
英文摘要:
      Quality properties of wheat (Triticum aestivum L.) are strongly affected by low molecular weight glutenin subunits (LMW GS). It is very important for improvement of wheat qualities to study the LMW GS compositions and distribution in Shaanxi wheat cultivars and advanced lines. STS markers were used to detect the allelic variations of LMW GS at Glu A3and Glu B3in 175 wheat cultivars and advanced lines from Shaanxi Province. Four allelic variations of LMW GS were detected at Glu A3locus in Shaanxi wheats, i.e. Glu A3a, Glu A3b, Glu A3cand Glu A3d, accounting for 12.6%, 1.7%, 58.3% and 27.4%, respectively. And eight allelic variations were found at Glu B3locus, i.e. Glu B3a, Glu B3b, Glu B3d, Glu B3e, Glu B3f, Glu B3g, Glu B3iand Glu B3j, accounting for 4.6%, 2.9%, 45.7%, 0.6%, 2.9%, 8.5%, 4.0% and 30.8%, respectively. There were different types and combinations of LMW GS allelic variations in three diverse regions of Shaanxi Province, which was likely related to the regional eating habits, wheat breeding programs and parental materials selected in wheat breeding.
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