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作者	单位
胡斯攀，邹宏达，郭来春，殷长强，刘宏魁，吴颖，张春生，任长忠，原亚萍	（1.吉林大学植物科学学院，吉林长春 130062； 2.吉林省白城市农业科学院，吉林白城 137000）

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中文摘要:

为了寻找燕麦光周期不敏感基因的分子标记，本研究采用SSR及AFLP标记对来自加拿大和中国的22份燕麦材料进行了DNA指纹分析及光周期不敏感基因的分子标记研究。结果表明，所选6对燕麦SSR引物中有3对扩增出多态性高的条带，通过带型分析可以将所研究的多数燕麦品种区分开，所选的10对大麦SSR引物和4对小麦SSR引物在22个燕麦品种中多态性不高，不能将不同燕麦品种区分开来。同时利用AFLP标记对其中5个不同熟期的燕麦材料进行了分析，64对AFLP引物组合经选扩共获得18 240个条带，平均57条/引物组合。统计分析AFLP图谱，获得品种特异条带20条，可将各燕麦品种区分开。此外获得光周期不敏感燕麦品种特异条带2条，可作为燕麦光周期不敏感基因的相关分子标记。

英文摘要:

To find molecular markers related to the photoperiod insensitivity genes in oat, a total of 22 oat cultivars from China and Canada which had different growth periods were studied on molecular marker by using SSR and AFLP. The results indicated that 3 pairs of 6 pairs SSR primers from oat showed high polymorphism among the 22 oat cultivars, while 10 pairs of SSR primers from barley and 4 pairs of SSR primers from wheat did not show high polymorphic . Most of the 22 oat cultivars can be distinguished by analysis of the SSR fingerprint which were amplified by the 3 pairs of SSR primers from oat, but not from barley and wheat. Then 5 oat cultivars having significant different growth periods were analyzed by AFLP. A total of 18,240 bands were detected by 64 AFLP primer combinations, and 57 bands per primer pair were amplified. According to the AFLP fingerprint, we obtained 20 species specific bands by which all the 5 oat cultivars could be distinguished. Otherwise, 2 specific bands were obtained from photoperiod insensitivity oat cultivars which could be related markers for the photoperiod insensitivity genes in oat.

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