王竹林,王艺桦,刘联正,奚亚军,刘曙东.小麦抗白粉病基因 Pm4的分子标记辅助育种研究[J].麦类作物学报,2011,31(5):819 |
小麦抗白粉病基因 Pm4的分子标记辅助育种研究 |
Molecular Marker Assisted Selection for Powdery Mildew Resistance Gene Pm4 in Wheat Breeding |
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DOI:10.7606/j.issn.1009-1041.2011.05.005 |
中文关键词: 小麦 白粉病 抗病基因 Pm4 分子标记辅助选择 |
英文关键词:Wheat Powdery mildew Resistance gene Pm4 Marker assisted selection |
基金项目:国家自然科学基金项目(30971768);西北农林科技大学唐仲英育种基金项目。 |
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中文摘要: |
Pm4是我国目前有效的小麦抗白粉病基因之一,该基因对陕西关中当前流行的白粉病菌系关中4号表现高抗。本研究利用与 Pm4基因紧密连锁的STS470分子标记对7个小麦品种间的6个杂交组合F3代单株进行分子标记检测并结合田间抗性鉴定筛选出抗病单株14个。对由这14个单株衍生出的64个F5代品系进行分子检测和田间抗性鉴定,发现53个品系表现抗病,占总品系的82.81%,其中51个抗病品系可以检测到与 Pm4基因紧密连锁的STS470特异带,占总品系的79.69%。本研究结果表明,STS470是 Pm4基因的可靠标记,可有效的用于分子标记辅助育种。 |
英文摘要: |
Pm4, a high resistant gene to the prevalent race Guanzhong 4 of powdery mildew pathogen, is one of the most effective wheat powdery mildew resistant gene in China. Molecular marker assistant selection is not limited by environment and cropping season, its application in the breeding program can improvement the accuracy of selection and breeding efficiency. In this study, 14 disease resistant F3 plants from six crosses of seven parents were selected by both STS470 marker and field disease test. Sixty four F3 lines, derived from the 14 F3 plants, were tested in the field and screened by molecular marker STS470. Among the 64 lines, there were 53 lines (82.8%) showed high resistance to the powdery mildew, of which 51 lines (79.7%) with resistant molecular marker STS470. The results showed that STS470 was a reliable molecular marker for screening Pm4 resistant gene and it could be effectively used in wheat breeding. |
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