| 李凤艳，徐兆师，李雅轩，晏月明，李连城，陈 明，马有志.小麦转录因子 TaDREB6基因启动子的克隆及活性分析[J].麦类作物学报,2011,31(5):793 |
| 小麦转录因子 TaDREB6基因启动子的克隆及活性分析 |
| Cloning and Activity Analysis of TaDREB6 Promoter in Wheat |
| |
| DOI：10.7606/j.issn.1009-1041.2011.05.001 |
| 中文关键词: 小麦 DREB 启动子 反向PCR 瞬时表达 |
| 英文关键词:Wheat DREB promoter Inverse PCR Transient expression |
| 基金项目:转基因生物新品种培育重大专项（2009ZX08009 083B、2009ZX08002 008B）；国家自然科学基金项目(31171546)。 |
|
| 摘要点击次数: 1678 |
| 全文下载次数: 1191 |
| 中文摘要: |
| 启动子的分析有助于解析植物抵御不良环境的机制。植物DREB转录因子参与对干旱、低温和高盐等胁迫的响应，在抗逆中起着重要作用。本研究利用反向PCR方法从小麦中克隆获得DREB转录因子TaDREB6基因启动子，长度为1 705 bp。PLACE和PlantCARE分析发现，TaDREB6基因启动子包含多种胁迫相关元件。构建由TaDREB6基因启动子驱动的GUS植物表达载体，转化小麦成熟胚愈伤组织，组织化学染色结果表明，TaDREB6基因启动子是诱导型启动子，受干旱、低温、盐、脱落酸和水杨酸等胁迫诱导。Real time PCR显示，TaDREB6基因受多种胁迫诱导表达，与TaDREB6启动子活性分析结果一致，这些结果为进一步分析DREB转录因子的功能提供了依据。 |
| 英文摘要: |
| DREB (dehydration responsive element binding) transcription factors response to drought, low temperature, and high salt etc and help plants survive in environmental stresses. To analyze the molecular mechanism of DREB , TaDREB6 promoter was cloned by inverse PCR from wheat genome, with full length of 1705 bp. Many basic cis acting elements related to various environmental stresses and plant hormones were found in the promoter sequence by PLACE and PlantCARE. To analyze the promoter activity, plant transient expression vector fused with the GUS (β glucuronidase) gene, driven by TaDREB6 promoter was constructed and transformed into mature embryo calli of wheat. The calli were treated by drought, ABA, high salinity, SA, and low temperature and gave transient expression at different degrees. The TaDREB6 expression levels increased when treated with drought, low temperature, ABA, SA and NaCl. These results indicated TaDREB6 promoter was stresses induced promoter, and help to illuminate DREB′s function in improving plants resistance. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
