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孙 一,胡亚亚,杨文香,刘大群.六个小麦品系的抗叶锈性评价[J].麦类作物学报,2011,31(4):762
六个小麦品系的抗叶锈性评价
Evaluation of the Resistance to Leaf Rust of 6 Wheat Lines
  
DOI:10.7606/j.issn.1009-1041.2011.04.032
中文关键词:  小麦  抗叶锈基因  基因推导  分子标记辅助选择
英文关键词:Wheat  Resistant gene to wheat leaf rust  Gene postulation  Molecular marker assisted selection
基金项目:国家“十一五”支撑计划项目(2006BAD08A05);国家公益性行业(农业)科研专项(200903035)。
作者单位
孙 一,胡亚亚,杨文香,刘大群 (河北农业大学植物病理学系 / 河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心, 河北保定 071001) 
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中文摘要:
      为探究供试的6个小麦品系对叶锈病的抗性状况及所含的抗叶锈基因,利用苗期基因推导、成株期抗病鉴定和分子标记辅助检测进行综合鉴定。结果表明,6个小麦品系中,cp02 62 8 1 1可能含有Lr3bgLr42Lr44;cp01 27 3 3 177可能含有Lr2cLr3bgLr16Lr26Lr42;cp20 3 3 4 178和cp02 39 2 4 377可能含有Lr16Lr26;cp20 30 1 5 218可能含有Lr3、 Lr3bgLr11Lr17Lr26;cp20 38 4 3 2具有很好的苗期和成株期抗性,可能含有Lr16Lr26Lr37和其他抗叶锈基因。测试的6份小麦材料不含有Lr1、 Lr9Lr10Lr12Lr19Lr20Lr21Lr24Lr28Lr29Lr34Lr35Lr38Lr41Lr47。结果表明,这些小麦品系中含有比较丰富的抗叶锈病基因,具有较好的抗叶锈性。
英文摘要:
      In order to evaluate the leaf rust resistance of 6 wheat lines tested, we did the resistance identification in seedling and adult stages with 42 resistance gene lines as tester, inoculated by 8 path types of pucciuina triticina. Combined gene postulation with the molecular assisted identification, we could concluded that Lr3ka, Lr42and Lr44 were posttulated in cp02 62 8 1 1; Lr2c, Lr3bg, Lr16, Lr26 and Lr42 were posttulated in cp01 27 3 3 177; Lr16 and Lr26 were present in cp20 3 3 4 178; Lr3,Lr3bg, Lr11, Lr17, Lr26 and Lr42 were present in cp20 30 1 5 218 and cp02 39 2 4 377. cp20 38 4 3 2 may carry Lr16, Lr26, Lr37 and other know or un known resistance genes to leaf rust. However, the specific bands for resistant genes of Lr1 Lr9, Lr10, Lr12, Lr19, Lr20, Lr21, Lr24, Lr28, Lr29, Lr34, Lr35, Lr38, Lr41 and Lr47 were not be detected with the corresponding primers in the 6 lines. The results indicated that there were quite rich resistant genes to leaf rust in the 6 wheat lines.
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