In order to evaluate the leaf rust resistance of 6 wheat lines tested, we did the resistance identification in seedling and adult stages with 42 resistance gene lines as tester, inoculated by 8 path types of pucciuina triticina. Combined gene postulation with the molecular assisted identification, we could concluded that Lr3ka, Lr42and Lr44 were posttulated in cp02 62 8 1 1; Lr2c, Lr3bg, Lr16, Lr26 and Lr42 were posttulated in cp01 27 3 3 177; Lr16 and Lr26 were present in cp20 3 3 4 178; Lr3,Lr3bg, Lr11, Lr17, Lr26 and Lr42 were present in cp20 30 1 5 218 and cp02 39 2 4 377. cp20 38 4 3 2 may carry Lr16, Lr26, Lr37 and other know or un known resistance genes to leaf rust. However, the specific bands for resistant genes of Lr1 Lr9, Lr10, Lr12, Lr19, Lr20, Lr21, Lr24, Lr28, Lr29, Lr34, Lr35, Lr38, Lr41 and Lr47 were not be detected with the corresponding primers in the 6 lines. The results indicated that there were quite rich resistant genes to leaf rust in the 6 wheat lines. |