Improving pre harvest sprouting (PHS) tolerance is one of the most important objectives in wheat breeding. In this study, 32 accessions of Triticum aestivum ssp. Yunnanense King (so called Yunnan Hulled Wheat), which was stored for 3 months under the -20℃ and room conditions after harvested, were employed to evaluate PHS tolerance and its components,respectively. In the intact spike experiment, the average germination ratio of Yunnan Hulled Wheat was 14.3%, and the germination degree was 6.0%, indicated that Yunnan Hulled Wheat had very strong tolerance to PHS.In the whole grain sprouting study, the average germination ratio was 24.3%, and the germination degree was 21.8%, suggesting they had very strong dormancy, and among those 2 accessions had the longest dormant period and 3 accessions had weak dormant ability. In the half grain experiment, the average germination ratio and index was 40.2% and 28.7%, respectively. In the glumes plus grain experiment, the average germination ratio and index was 11.8% and 9.4%, respectively.These indicated that inhibitor exuded from the glumes had strong inhibition on grain germination. As for the material stored under the room condition, the germination ratio reduced very quickly from 27.4% of the first day to 1.9% of the fifth day, until 0.6% of the seventh day, eventually there were 34.1% grains fail to sprout, these results indicated that along with the experimental time, the inhibitor from the glumes accumulated and the effect of inhibition increased. The tolerance of Yunnan hulled wheat to the PHS mostly depended on the seed dormancy, and then the inhibition effect of tenacious and tight glumes, while the inhibitor exuded from the glumes also had a great contribution to pre harvest sprouting. |