| 刘 莉，陈云芳，杨文香，闫红飞，刘大群.小麦抗叶锈基因 Lr20、 Lr28、 Lr29的STS、SCAR标记在近等基因系上的特异性验证[J].麦类作物学报,2011,31(3):402 |
| 小麦抗叶锈基因 Lr20、 Lr28、 Lr29的STS、SCAR标记在近等基因系上的特异性验证 |
| Verification of STS and SCAR Molecular Markers for Leaf Rust Resistance Genes Lr20, Lr28 and Lr29 in Near isogenic Lines of Wheat cv.Thatcher |
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| DOI：10.7606/j.issn.1009-1041.2011.03.003 |
| 中文关键词: 小麦 叶锈 近等基因系 抗叶锈病基因 STS分子标记 SCAR分子标记 |
| 英文关键词:Wheat Leaf rust Near isogenic lines Resistance genes STS molecular marker SCAR molecular marker |
| 基金项目:国家公益性行业（农业）科研专项（200903035）；国家自然科学基金项目（30771391）。 |
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| 中文摘要: |
| 为了把特异性分子标记与抗病性鉴定有效地结合起来，用含有不同抗叶锈基因的54个以Thatcher为遗传背景的近等基因系（Near isogenic lines，NILs）对与抗叶锈基因 Lr20、 Lr28和 Lr29（ Lr29UBC和 Lr29OPY）连锁的STS、SCAR标记进行特异性验证，结果分别扩增出片段大小依次为540、378、1 000、900 bp的条带，与报道片段大小一致。同时发现与抗病基因 Lr20、 Lr29连锁的STS分子标记及与 Lr29连锁的两个SCAR标记 Lr29UBC、 Lr29OPY在NILs中特异性较好，但 Lr28的PCR STS Lr28扩增产物不仅在其亲本中，而且在其余53个近等基因系中都扩增出与报道大小相同的378 bp的条带。验证结果表明，与抗病基因 Lr20、 Lr29连锁的STS、SCAR分子标记在NILs中特异性较好，可方便地用于小麦抗叶锈的分子标记辅助选择育种，而 Lr28的STS分子标记没有特异性，不能用于分子标记辅助选择育种。 |
| 英文摘要: |
| Sequence tag site (STS) and SCAR markers for genes Lr20, Lr28, Lr29（ Lr29OPY and Lr29UBC）against Puccinia triticina were used to screen a set of near isogenic lines (NILs) of wheat cv. Thatcher, which containing in total 54 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis using STS and SCAR primers specific for these leaf rust resistance genes indicated the marker loci of 540 bp, 378 bp, 900 bp and 1 000 bp could be obtained as the same as reported previously. The result showed the loci of markers STS Lr20 STS638, SCAR Lr29 UBC and SCAR Lr29 OPY were unique and specific, and could be used for molecular marker assisted selection. But the locus of the STS Lr28 marker for Lr28 was detected in all the near isogenic lines used in this study, which indicated that the locus of marker STS Lr28 was not specific, and could not be used for molecular marker assisted selection. |
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