| 邓秀敏，杜俊变，段江燕.UV B辐射增强对小麦叶片蛋白质含量的影响[J].麦类作物学报,2011,31(2):154 |
| UV B辐射增强对小麦叶片蛋白质含量的影响 |
| Preliminary Analysis on the Effects to Wheat Leaves Proteins by Enhanced UV B Radiation |
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| DOI：10.7606/j.issn.1009-1041.2011.02.027 |
| 中文关键词: 小麦 UV B辐射 蛋白质 |
| 英文关键词:Wheat UV B radiation Protein |
| 基金项目:山西省自然科学基金项目（2009011044 2）。 |
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| 中文摘要: |
| 为了解UV B辐射增强对小麦叶片蛋白质的影响，采用双向电泳技术分析了UV B辐射增强处理后小麦叶片蛋白质的变化。结果表明，UV B辐射增强处理小麦幼苗6 d后，叶片总蛋白含量与对照差异极显著（P＜0.01）。经过双向电泳分析，UV B辐射增强处理与对照相比，差异在2倍以上的点有21个。其中上调表达的蛋白点有6个，下调表达的蛋白点有12个，新出现的蛋白点有3个。说明UV B辐射增强对蛋白总量及其表达均产生影响。 |
| 英文摘要: |
| In order to investigate the influence of enhanced UV B radiation to wheat , two dimensional electrophoresis were used to detect the changes of proteins in wheat leaves treated by enhanced UV B radiation. Results showed that at six days after enhanced UV B radiation treatment, there were significant difference (P＜0.01) in the total protein content between the UV B radiation treatment and control. Two dimensional electrophoresis analysis revealed that there were 21 protein points with over 2 times difference comparing that of the UV B radiation treatment with control, among which 6 were upregulated, 12 were downregulated, and 3 were new, this suggested enhanced UV B radiation influenced the expression of proteins in wheat. |
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