| 周 博，陈新宏，庞玉辉，赵继新，武 军，程雪妮，刘淑会.滨麦HMW GS启动子和编码区基因的分离与序列分析[J].麦类作物学报,2011,31(2):14 |
| 滨麦HMW GS启动子和编码区基因的分离与序列分析 |
| Isolation and Sequence Analysis of the HMW GS Promoter and ORF Genes from Leymus mollis |
| |
| DOI：10.7606/j.issn.1009-1041.2011.02.003 |
| 中文关键词: 滨麦 HMW GS 启动子 编码区 序列分析 |
| 英文关键词:Leymus mollis HMW GS Promoter Coding region Sequence analysis |
| 基金项目:国家自然科学基金项目(30771345)；教育部“新世纪人才支持计划” 项目(NCET 06 0862)；陕西省“13115”科技创新工程计划项目（2009ZDKG 11，2010ZDKG 08）。 |
|
| 摘要点击次数: 1737 |
| 全文下载次数: 1790 |
| 中文摘要: |
| 为了深入了解滨麦（Leymus mollis）HMW GS基因的结构特征，采用PCR方法，从滨麦基因组中分离出HMW GS基因启动子序列（Genbank登录号：FJ600498）和编码区序列（Genbank登录号：GQ169797）。启动子序列（FJ600498）从5′至3′方向依次有E box、N box、G box、HMW谷蛋白特异增强子和TATA box等麦谷蛋白特异调控元件，推断其为滨麦HMW GS启动子基因。编码区序列（GQ169797）具有单一完整的开放阅读框（ORF），编码396个氨基酸残基，依次包含信号肽、N 末端区、中部重复区和C 末端区等HMW GS的典型结构区域；中部重复区主要重复单元为6肽（PQQGQQ）和9肽（GYYPTSPQQ）；有6个半胱氨酸残基（Cys），分布在N 末端区（5个）和C 末端区（1个），第3、4个相邻。推断其为滨麦的y 型HMW GS编码区基因。系统进化分析表明，启动子序列（FJ600498）与异形花草（He. piliferum）和冰草（Ag. cristatum）的HMW GS启动子基因序列具有相对较近的同源关系；编码区序列（GQ169797）与纤毛鹅观草（Elymus ciliaris）和披碱草（E. glaucus）的HMW GS编码区基因序列具有相对较近的同源关系。 |
| 英文摘要: |
| HMW GS promoter sequence (FJ600498) and coding region sequence (GQ169797) were isolated from the genome of L.mollis by the method of PCR. The HMW GS promoter sequence of L.mollis from 5′ to 3′ flanking in turn contained E box, N box, G box, 38bp HMW glutenin specific enhancer and TATA box, which were the promoter regulatory elements of typical HMW GS gene. The HMW GS coding region sequence had a single complete open reading frame (ORF), which encoding 396 amino acids. The deduced amino acid sequence contained the signal peptides, N terminal region, the central repeat region, and C terminal region. In repeat domains, hexapeptide (PGQGQQ) and nonapeptide (GYYPTSPQQ) were the main repeating unit. The sequence had six cysteine residues, five within N terminal and one within C terminal domains, and the 3rd and the 4th cysteine neighboring. These structure characteristics suggested that GQ169797 is a y type HMW GS gene of L.mollis. Phylogenetic analysis showed that the promoter (FJ600498) was closely related with the HMW GS promoter sequence of Heteranthelium piliferum and Agropyron cristatum and the ORF sequence has a relatively close homology with y type HMW GS gene of Elymus ciliaris and Elymus glaucus. |
|
查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
基本信息
