| 邱志刚，刘 沛，翟朝增，徐兆师，李连城，陈 明，马有志.小麦TaBS1转录因子基因的克隆、原核表达及Western blot检测[J].麦类作物学报,2011,31(2):1 |
| 小麦TaBS1转录因子基因的克隆、原核表达及Western blot检测 |
| Isolation, Prokaryotic Expression and Western Blot of Transcription Factor TaBS1 Gene from Wheat |
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| DOI：10.7606/j.issn.1009-1041.2011.02.001 |
| 中文关键词: 小麦 转录因子 原核表达 Western blot |
| 英文关键词:Wheat Transcription factor Prokaryotic expression Western blot |
| 基金项目:国家自然科学基金项目（30700504）；转基因生物新品种培育重大专项（2009ZX08009 083B，009ZX08002 008B）。 |
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| 中文摘要: |
| 转录因子在提高植物抗性中起着重要的作用。本实验从小麦中克隆得到一个新的具有DUF822保守域的转录因子基因 TaBS1。为进一步研究该转录因子在参与植物胁迫信号调控中的作用以及蛋白本身的结构、功能和活性等，将 TaBS1基因构建到原核表达载体pET 28a(+)上。在终浓度为1 mmol·L-1 IPTG诱导2 h的条件下，得到融合蛋白 His TaBS1，并用Western blot确定所表达的蛋白确实为融合蛋白 His TaBS1。 |
| 英文摘要: |
| Transcription factors play an important role in improving resistance in plants. A novel transcription factor gene, named TaBS1, was isolated from wheat.In order to investigate the role of TaBS1 in response to stresses and the structure, function and activity of proteins, the TaBS1 gene was constructed into the prokaryotic expression vector pET 28a(+). The fusion protein His TaBS1was induced to expression using 1 mmol/L of IPTG.The fusion protein His TaBS1 was detected by Western blot. This study laid a basis for investigation of protein function, activity, and structure ofTaBS1. |
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