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为研究小麦的耐盐机理，在以前的工作中，本课题组采用cDNA AFLP技术获得一个219 bp的泛素羧基末端水解酶的片段，本研究以该片段为基础，通过电子延伸、RACE和RT PCR，得到了两个全长cDNA克隆，分别命名为TaUCH1和TaUCH2，这两条序列在编码区只有一个氨基酸的差异。保守结构域预测显示，TaUCH1和TaUCH2的中部和C末端各有一个C19肽酶结构域。将TaUCHs及其在植物中的同源蛋白进行对比发现，TaUCHs与水稻中的同类蛋白相似性最高，有88%的同源性。Southern杂交显示，TaUCHs在小麦中以多拷贝或基因家族形式存在。以上结果说明TaUCHs是小麦中的羧基末端水解酶基因，可能参与了植物胁迫反应中某些受损伤蛋白或重要调控蛋白的降解。

英文摘要:

In order to study the mechanism of salt tolerance in plants, a 219 bp cDNA fragment sharing high similarity with ubiquitin C terminal hydrolase was obtained by cDNA AFLP in previous work. Based on this fragment, two full length cDNAs were cloned via silico cloning, RACE and RT PCR, and named as TaUCH1 and TaUCH2 respectively. They are almost identity except for an amino acid difference in the open reading frame. Conserved domain prediction indicated that there are C19 peptidase domains both in the middle and at the C terminal of the two proteins. Phylogenetic analysis indicated that TaUCH1 and TaUCH2 share 88% similarity with their homologous proteins in rice. Southern blot revealed that TaUCHs consist of multiple copies or a gene family in wheat.These results indicated that TaUCHs are the real ubiquitin C terminal hydrolase genes in wheat and may involve in the degradation of some damaged proteins or important regulators during plant stress.

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