谭彩霞,封超年,郭文善,朱新开,李春燕,彭永欣.缺失不同Wx蛋白对小麦籽粒直链淀粉合成及淀粉特性的影响[J].麦类作物学报,2010,30(5):920 |
缺失不同Wx蛋白对小麦籽粒直链淀粉合成及淀粉特性的影响 |
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DOI:10.7606/j.issn.1009-1041.2010.05.025 |
中文关键词: 小麦 Wx蛋白 直链淀粉 GBSSI GBSS |
英文关键词:Wheat Wx protein Amylose Granule bound starch synthase gene Granule bound starch synthase |
基金项目:江苏省“六大人才高峰”项目(07 G 008);国家自然科学基金项目(31071340,30370829);扬州大学自然科学基金项目(2007cxj015)。 |
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中文摘要: |
为探索糯小麦籽粒直链淀粉合成的生理机制,以缺失不同Wx蛋白的小麦品种为试验材料,研究缺失不同Wx蛋白对小麦籽粒直链淀粉合成及淀粉特性的影响。结果表明,缺失不同Wx蛋白对小麦籽粒直链淀粉含量、积累量和积累速率、束缚态淀粉合成酶基因(GBSSI)相对表达量以及束缚态淀粉合成酶(GBSS)活性的影响顺序均表现为:ABD型(缺失Wx A1、Wx B1、Wx D1)>AB型(缺失Wx A1和Wx B1)>B型(缺失Wx B1)>D型(缺失Wx D1)>A型(缺失Wx A1)>正常型;对淀粉最终黏度、反弹值以及糊化温度影响的表现顺序与其一致;对淀粉峰值黏度、低谷黏度、稀懈值以及膨胀势影响的表现顺序与此相反。 |
英文摘要: |
The purposes of the present study with wheat missing different Wx proteins as tested materials were to investigate the effect of missing different Wx proteins on amylose synthesis and starch properties in wheat grains. The results showed that: the effects of missing different Wx protein on amylose content, expression level of GBSSI and GBSS activity were recorded in the following order: waxy type>double null allels of Wx A1 and Wx B1>single null allels of Wx B1>single null allels of Wx D1 > single null allels of Wx A1>normal type. Similar trends were observed in final viscosity, rebound value, and gelatinization temperature, while opposite trends were measured in peak viscosity, trough viscosity, breakdown and expansion. |
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