| 杨汉波，胡 蓉，王春艳，王 芳，李 清，段辉国.重金属Pb2+、Cd2+胁迫对青稞幼苗抗氧化能力的影响[J].麦类作物学报,2010,30(5):842 |
| 重金属Pb2+、Cd2+胁迫对青稞幼苗抗氧化能力的影响 |
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| DOI：10.7606/j.issn.1009-1041.2010.05.011 |
| 中文关键词: 青稞幼苗 Pb2+、Cd2+胁迫 抗氧化能力 |
| 英文关键词:Hulless barley seedling Pb2+ and Cd2+ stress Antioxidative ability |
| 基金项目:四川省教育厅重点研究项目（09ZA054）；内江师范学院大学生科研项目（08NSD 128）；内江师范学院生态学重点建设学科项目。 |
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| 摘要点击次数: 1898 |
| 全文下载次数: 1825 |
| 中文摘要: |
| 为了解重金属对青稞幼苗的伤害机理以及青稞对重金属胁迫的抗性作用，以青稞幼苗为实验材料，采用水培方法研究了50 mg·L-1 Pb2+、Cd2+胁迫对青稞幼苗叶片相对含水量（RWC）、丙二醛（MDA）和脯氨酸含量、抗氧化酶活性的影响。结果表明，50 mg·L-1 Pb2+ 胁迫下青稞幼苗的叶片相对含水量（RWC）变化不大，丙二醛（MDA）和脯氨酸含量、过氧化物酶（POD）和过氧化氢酶（CAT）活性在胁迫后期有所升高，超氧化物歧化酶（SOD）活性在前期升高比较明显，其后缓慢下降。50 mg·L-1 Cd2+胁迫下青稞幼苗叶片RWC明显减少，MDA含量显著增加，脯氨酸含量先急剧升高后下降，POD、CAT活性随着胁迫时间的增加而显著增强，其中CAT活性在胁迫末期稍有下降，SOD活性先明显升高后下降。综合来看，青稞幼苗在受到相同浓度Pb2+、Cd2+胁迫时，对Pb2+的抵抗能力大于Cd2+。 |
| 英文摘要: |
| The effects of 50 mg·L-1 Pb2+ and Cd2+ stress on relative water content(RWC), malondialdehyde (MDA), proline contents and activity of antioxidant enzyme in hulless barley seedlings were investigated. The results showed that RWC changed little, MDA and proline contents, peroxidase (POD) and catalase (CAT) activity increased, superoxide dismutase (SOD) activities increased at first, then decreased slowly in leaves of hulless barley seedlings under 50 mg·L-1 Pb2+stress. RWC reduced significantly, MDA content increased significantly, proline content increased significantly at first, then decreased, activities of POD and CAT increased continually, SOD activities increased markedly at first, then decreased in leaves of hulless barley seedlings under 50 mg·L-1 Cd2+stress. These results suggested that resistance to Pb2+was larger than resistance to Cd2+ in hulless barley seedlings under stress of the same concentration Pb2+ and Cd2+. |
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