| 曹 莉，王 辉，孙道杰，冯 毅，李学军，闵东红.小麦黄化突变体类囊体蛋白组分及叶绿素的合成特性[J].麦类作物学报,2010,30(4):638 |
| 小麦黄化突变体类囊体蛋白组分及叶绿素的合成特性 |
| Chloroplast Thylakoid Protein Composition and Characteristics of Chlorophyll Biosynthesis in a Novel Aurea Mutant of Wheat |
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| DOI：10.7606/j.issn.1009-1041.2010.04.010 |
| 中文关键词: 小麦 黄化突变体 类囊体蛋白 叶绿素合成 |
| 英文关键词:Wheat Aurea mutant Chloroplast thylakoid protein Chlorophyll biosynthesis |
| 基金项目:农业部“948”项目“分子育种实用化研究”（2006G2）；国家“973”计划项目“小麦高产优质品种设计和选育的应用基础研究”（2009CB118300）。 |
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| 中文摘要: |
| 为进一步探讨冬小麦品系“西农1718”自然黄化突变体的突变机制，利用SDS 尿素 聚丙烯酰胺凝胶电泳对该黄化突变体及其突变亲本叶绿体类囊体蛋白组分进行比较研究。结果表明， 与突变亲本相比，黄绿株（黄化程度较轻的中间型）的60～64 kD和42～54 kD多肽无明显变化，而绿黄株（黄化程度较深的中间型）和金黄株显著减少，且金黄株减少幅度大于绿黄株；黄绿株、绿黄株的33～35 kD多肽表达量明显低于突变亲本，且绿黄株减少幅度大于黄绿株；23和27 kD多肽在突变体中的变化最为显著，金黄株缺失，黄绿株和绿黄株也大幅降低；16～19 kD多肽，黄绿株与突变亲本无明显差异，金黄株和绿黄株大幅度降低。对叶绿素生物合成主要前体物质累积量分析表明，金黄株、绿黄株和黄绿株的δ 氨基乙酰丙酸（ALA）、胆色素原（PBG）、尿卟啉III（Urogen Ⅲ）、粪卟啉原 Ⅲ（Coprogen Ⅲ）和原卟啉Ⅸ（Proto Ⅸ）的含量均高于突变亲本，且随突变体黄化程度加深而递增，尤其Proto Ⅸ累积更为显著，分离绿株与突变亲本无差别；而金黄株、绿黄株和黄绿株自Proto Ⅸ以后的中间产物镁原卟啉（Mg Proto Ⅸ）、原脱植基叶绿素酸酯（Pchlide）的含量均显著低于突变亲本，且随突变体黄化程度加深而递减，分离绿株与突变亲本无明显差异。因此初步认为，该黄化突变体叶绿素合成受阻，且受阻位点发生在Proto Ⅸ到Mg Proto部位。 |
| 英文摘要: |
| Chloroplast thylakoid protein were separated by fully denaturing SDS PAGE electrophoresis. As compared with wild type, there were no significant changes in yellowish green plant and significant reduction in greenish yellow and aurea plant in the composition of 60~64 kD and 42~54 kD polypeptide. The expression level of 33 ~ 35 kD polypeptide of greenish yellow and yellowish green were significantly lower than wild type, and greenish yellow reduced greater than yellowish green. Particularly, 23 kD and 27 kD polypeptides were greatly diminished in amount in greenish yellow and yellowish green plants, and they were absent in aurea plant. With the deepening of yellow, the contents of 16 ~ 19 kD polypeptides significantly reduced, no significant differences between yellowish green plant and wild type, but in aurea plant and greenish yellow. The major precursors of chlorophyll biosynthesis were analysed and showed that the content of ALA, PBG, UrogenIII, Coprogen and Proto Ⅸ were all over than that of the wild type; in contrast, Mg Proto and Pchlide were all decreased than wild type. This result indicated Coprogen and protoporphyrin accumulated in the mutant, and the rate of conversion of Proto Ⅸ to Mg Proto Ⅸ was very lower than that of wild type. It is concluded that, the mutation brings about a restriction in the rate of conversion of protoporphyrin to magnesium protoporphyrin; and in the mutant, the enzymes converting protoporphyrin into Mg Proto Ⅸ were most likely absent or damaged. |
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