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为了探索小麦Mn SOD基因的克隆及表达，根据GenBank上公布的序列(AF092524)设计引物，利用Reverse transcriptase Polymerase Chain Reaction（RT PCR）技术克隆了小麦Mn SOD基因的cDNA全长，并发现该基因主要在根中表达，在其他组织中的表达量比较低。通过不同浓度的NaCl溶液处理，发现该基因在小麦的根中可以被诱导调控表达。随着NaCl浓度的提高，该基因的表达量持续增强；但是NaCl的浓度超过0.20 mol·L^-1时，其表达量反而下降。

英文摘要:

Superoxide dismutase plays a key role in resisting various stress of plants. In this study, we cloned the full length of the Mn SOD gene according to the sequence released in the GenBank of NCBI. Through reverse transciptase Polymerase Chain Reaction (RT PCR), we found this gene mainly expressed in roots of wheat, and, its expression level was very low in other tissues. In order to understand the relationship between the expression of the gene and stress plants suffered, we treated wheat seedlings with various content of NaCl. As a result, the expression level sufficiently increased in roots of seedlings treated with NaCl compared with control seedlings. As the content of NaCl becoming higher, the expression level of the gene increased constantly. But when the content of NaCl was higher than 0.20 mol·L^-1，its expression would decrease significantly even lower than control seedlings. This research will be beneficial to strengthening ability of wheat against water stress with gene engineering technology.

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