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作者	单位
王正阳1，倪永静1，牛吉山1 ，刘靖2	1. 河南农业大学国家小麦工程技术研究中心，河南郑州 450002； 2. 河南农业大学生命科学院，河南郑州 450002

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中文摘要:

为了解目前普通小麦育成品种（系）间醇溶蛋白的遗传多样性，利用酸性聚丙烯酰胺凝胶电泳（Acid polyacrylamide gel electrophoresis, A PAGE）技术对国内99份小麦新品种（系）进行了醇溶蛋白分析。结果表明，供试材料中共分离出蛋白带2 192条，迁移率不同的谱带类型106种，其中迁移率编号为9和11的2种谱带出现频率最高，均为82.11%；有38条谱带的出现频率低于10%，这些低频率谱带仅出现在极少数的材料中；其余66条谱带出现频率为10.53%~64.21%，具有较高的多态性。每个被检测材料可电泳分离出15 ~ 30条带，其中大部分为18 ~ 24条。供试材料间遗传距离（Genetic distance, GD）为0.07 ~ 0.73，平均为0.59，遗传变异较大。聚类分析可将其分为4大类。与过去的小麦品种醇溶蛋白研究结果相比，新品种（系）的平均遗传距离缩小，遗传基础变窄。

英文摘要:

In order to investigate the genetic diversity at gliadin loci in new wheat (Triticum aestivum L.) cultivars or lines, and to provide genetic information for parent selection in wheat breeding program, 99 new domestic wheat cultivars or lines were analyzed by acid polyacrylamide gel electrophoresis (A PAGE). The results showed that a total of 2 192 protein bands were identified with 106 different relative mobility rates in the examined wheat accessions. Both bands of No. 9 and 11 had the highest present frequency of 82.11%, 38 bands had low present frequencies below 10%, and the other 66 bands had frequency of 10.53% ~64.21% with higher polymorphisms. Each accession had 15 to 30 bands, most of them ranged from 18 to 24. The genetic distances (GD) of the examined materials varied from 0.07 to 0.73, with an average of 0.59. All the examined accessions could be divided into 4 clusters. Compared with the genetic diversity at gliadin loci in wheat germplasms reported previously, the genetic distance was becoming less between new cultivars or lines, genetic base of new lines was relative narrow.

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