| 金广慧，杜向红，谭秀芳，高永钢，宋卫宁.硬粒小麦和野生二粒小麦高分子量谷蛋白亚基的遗传多样性[J].麦类作物学报,2010,30(2):227 |
| 硬粒小麦和野生二粒小麦高分子量谷蛋白亚基的遗传多样性 |
| Genetic Diversity of High Molecular Weight Glutenin in Durum Wheat and Wild Emmer Wheat |
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| DOI：10.7606/j.issn.1009-1041.2010.02.008 |
| 中文关键词: 野生二粒小麦 硬粒小麦 高分子量谷蛋白亚基 遗传多样性 |
| 英文关键词:Durum wheat Wild emmer wheat High molecular weight glutenin Genetic diversity |
| 基金项目:教育部科技创新工程重大项目培育基金项目(707054)；高等学校学科创新引智计划项目（111 2 16）；国家“863”计划项目（2006AA100223）。 |
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| 中文摘要: |
| 为了解硬粒小麦和野生二粒小麦的高分子量谷蛋白亚基（HMW GS）组成及优质亚基的分布特点，并对其多样性进行分析，利用SDS 聚丙烯酰胺凝胶电泳（SDS PAGE）对32份硬粒小麦和75份野生二粒小麦共107份材料的HMW GS组成进行了分析。结果表明，Glu 1位点共有27种等位基因，其中Glu A1位点4种，Glu B1位点23种；亚基null和6+8在各自的位点上出现频率最高，分别达到38.84％和16.82％；其亚基组成类型共有47种，主要为1/6+8，频率达7.48％；同时筛选出33份含有1、2*、13+16、14+15、17+18等优质亚基的材料，可作为优质基因源。多样性分析结果表明，在Glu A1和Glu B1位点以及HMW GS组成上，野生二粒小麦的多样性都大于硬粒小麦。另外，在材料GW 2、GW 4中发现未命名的新亚基。 |
| 英文摘要: |
| In order to know the genetic diversity of high molecular weight gluten, 32 durum wheat and 75 wild emmer wheat were analyzed by means of SDS PAGE. The result indicated that there were 27 alleles on Glu 1 loci, including 4 alleles on Glu A1 loci, 23 alleles on Glu B1 loci; And null, 6+8 subunits occurred with highest frequency on each loci, reaching 38.84% and 16.82%, respectively. There were 47 types of subunit compositions and the predominant was 1/6+8 with a frequency of 7.48%. Materials conferred with elite subunits of 1, 2*, 13+16, 14+15, 17+18 were selected and could be a source of desirable genes. Results from the diversity analysis showed that the diversity of wild emmer wheat were greater than of durum wheat in the Glu A1 and Glu B1 loci, as well as HMW GS composition. One new subunit combinations type was found in wild emmer wheat materials GW 2 and GW 4. |
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