In the nutrient solution culture conditions, the effects of exogenous putrescine on membrane lipid peroxidation and antioxidant enzyme activity in wheat seedling leaves were investigated under 15% PEG 6000 drought stress. The results indicated that at 48 h after drought stress, content of water in wheat seedling leaves decreased by 27.43%, superoxide (O2) production rate, MDA and H2O2 content increased, while the SOD, POD, CAT activities decreased by 23%, 37%, 70%, respectively. Compared with drought stress, content of water in wheat seedling leaves only decreased by 7.62% in the treatment of 100 μmol·L-1 putrescine, accumulation of MDA declined about 80%, content of H2O2 declined about 50%,and O2 production rate reduced by 35%; however, SOD、CAT and POD activities increased by 41%, 165.8%, 246% in winter wheat seedling leaves. It indicated that exogenous putrescine alleviate membrane oxidative damage in winter wheat seedlings possible by inhibiting reactive oxygen species (O2, H2O2) production, decreasing content of MDA and enhancing the antioxidant enzyme (SOD, POD, CAT) activity, to improve drought resistance of winter wheat. |