李绍祥1,2,顾 坚2,刘 琨2,田玉仙2,杨和仙2,杨木军2,倪中福1.小麦温光敏雄性核不育系K78S不育性的遗传研究[J].麦类作物学报,2009,29(6):971 |
小麦温光敏雄性核不育系K78S不育性的遗传研究 |
Genetic Study on the Sterility of Thermo photo sensitive Genic Male Sterile Wheat K78S |
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DOI:10.7606/j.issn.1009-1041.2009.06.006 |
中文关键词: 小麦 温光敏雄性核不育性 遗传 |
英文关键词:Thermo photo sensitive genic male sterility Inheritance |
基金项目:国家自然科学基金项目(30560077)。 |
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中文摘要: |
K78S是以重庆温光敏小麦核不育系C49S 87转育的新不育系。为研究其不育性的遗传特性,利用组合K78S/1069和C49S 87/1069的杂种F1构建了2个DH群体即DH 1和DH 2群体,并对群体内各DH株系的育性进行了2个播期(2007年10月15日和10月25日)的重复鉴定。结果表明,DH 1和DH 2群体在2个播期间套袋结实率的相关系数分别为0.8050**和0.6828*,育性鉴定结果具有较好的一致性;通过曲线拐点法和卡方测验分析,DH 1的结实率在2个播期均表现为1∶3∶3∶9的分离比,表明K78S的不育性由2对隐性基因控制;DH 2在第1播期也表现为1∶3∶3∶9的分离比,但第2播期由于不育系C49S 87(套袋结实率达到17.1%)及其DH群体的不育性部分恢复,表现为1∶15的分离比,表明C49S 87的不育性仍由2对隐性基因控制。此外,DH 1、DH 2的套袋结实率分别在0~99.1%和0~98.8%的范围内均有分布,只是频数不同,因此不排除有微效多基因参与不育性的表达。 |
英文摘要: |
K78S is a new sterile line transferred from C49S 87, a thermo photo sensitive genic male sterile wheat line introduced from Chongqing. In order to understand the inheritance of sterility of K78S, two DH (doubled haploid) populations DH 1 and DH 2, separately derived from F1 of K78S/1069 and C49S 87/1069, were constructed with wheat×maize system. And the fertility of DH plants was investigated in two sowing dates (Oct. 15 and Oct. 25, 2007). The correlation coefficients of bagged seed set rates for DH 1 and DH 2 between both sowing dates were 0.8050** and 0.7066*, respectively. Curve flexion and Chi square analyses indicated that the observed segregation of seed set rates for DH 1 fits to a 1∶3∶3∶9 ratios in both sowing dates, suggesting the male sterility of K78S was controlled by two segregate recessive genes, the same result was got in first sowing date of DH 2, while in the second sowing, the ratio changed to 1∶15 because of the partial restoring of fertility for C49S 87 and the individuals, which meant two recessive genes were involved in the express of fertility of C49S 87. In addition, despite the difference in frequency distribution, the bagged seed set rates for DH 1 and DH 2 varied at 0~99.1% and 0~98.8%, respectively, indicating minor genes also influence the fertility of K78S and C49S 87. |
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