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作者	单位
张文斌1，任向辉1，安德荣1，何振才2	1. 西北农林科技大学植保学院，陕西省农业分子生物学重点实验室，陕西杨凌712100； 2. 陕西省杂交油菜研究中心小麦研究室，陕西大荔 715105

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中文摘要:

为明确病毒抑制剂对小麦黄矮病的预防和防治效果，在小麦起身期将大麦黄矮病毒GAV株系接种于小麦陕恳9740上，喷施杀虫剂（2.5%吡虫啉可湿性粉剂2000倍液）与病毒抑制剂（20%病毒A可湿性粉剂500倍液、3.95%病毒必克可湿性粉剂500倍液、5%菌毒清水剂500倍液和2%菌克毒克水剂300倍液）的不同组合，于小麦开花期统计发病率、病情指数和小麦叶片黄化率，结果表明，各组合均能早期预防和防治小麦黄矮病发生和发展，且预防效果优于防治效果，其中病毒必克与吡虫啉组合的平均预防和防治效果最显著，分别为58.08%和53.03%。进一步利用病毒必克在室内进行防治性试验，发现病毒必克能够抑制GAV对叶绿素的破坏，可减轻大麦黄矮病毒对小麦的危害。

英文摘要:

The efficacy trial was conducted in the field with wheat variety Shanken 9740, inoculating strain GAV of barley yellow dwarf virus(BYDV) with Schizaphis graminum were conducted at wheat setting up stage. Agrochemicals combination of pesticide 2.5% imidacloprid wettable power at a dilution of 2000 and plant virus inhibitors (20% Virus A wettable power at a dilution of 500, 3.95% Bilken virucide wettable power at a dilution of 500, 5% Junduqing aqueous solution at a dilution of 500 and 2% Ningnanmycin aqueous solution at a dilution of 300) were sprayed, incidence and disease index of wheat yellow dwarf as well as the number of yellowing leaf were investigated at flowering stage, the results showed that all combinations have preventive and control effect on accidence and development of wheat yellow dwarf, and the preventive effect is superior to the control effect. Among the above combinations, the effect of the combination of Bilken viruaside and imidacloprid was the best, and the average preventive and control effect were 58.08% and 53.03%，respectively. Then, chlorophyll in wheat leaves, extracted by mixed soak with analytically pure acetone and analytically pure ethanol (2∶1), were measured to test which wheat yellow dwarf was controlled by 3.95% Bilken virucide wettable power at a dilution of 500 in laboratory, the result indicated that Bilken viruaside could inhibit BYDV from the harm of chlorophyll to relieve the damage.

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